Characterization of BshA, bacillithiol glycosyltransferase from Staphylococcus aureus and Bacillus subtilis.

Characterization of BshA, bacillithiol glycosyltransferase from Staphylococcus aureus and Bacillus subtilis.
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DOI:
10.1016/j.febslet.2012.02.028
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发表时间:
2012-04-05
期刊:
影响因子:
3.5
通讯作者:
Rawat M
Rawat M
中科院分区:
生物学3区
文献类型:
--
作者:
Upton H;Newton GL;Gushiken M;Lo K;Holden D;Fahey RC;Rawat M

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The first step during bacillithiol (BSH) biosynthesis involves the formation of N-acetylglucosaminylmalate from UDP-N-acetylglucosamine and L-malate and is catalyzed by a GT4 class glycosyltransferase enzyme (BshA). Recombinant Staphylococcus aureus and Bacillus subtilis BshA were highly specific and active with L-malate but the former showed low activity with D-glyceric acid and the latter with D-malate. We show that BshA is inhibited by BSH and similarly that MshA (first enzyme of mycothiol biosynthesis) is inhibited by the final product MSH.
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