Hydrogen isotopic enrichment: an indicator of biodegradation at a petroleum hydrocarbon contaminated field site.
Hydrogen isotopic enrichment: an indicator of biodegradation at a petroleum hydrocarbon contaminated field site.
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DOI：
10.1021/es011253a
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发表时间：
2002-06
影响因子：
11.4
通讯作者：
S. Mancini;G. Lacrampe-Couloume;H. Jonker;B. V. van Breukelen;J. Groen;F. Volkering;B. S. Lollar
中科院分区：
文献类型：
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作者：
S. Mancini;G. Lacrampe-Couloume;H. Jonker;B. V. van Breukelen;J. Groen;F. Volkering;B. S. Lollar
采用碳、氢同位素分析方法研究了陶氏比荷卢工业区污染地下水中苯和甲苯的生物降解。与源区样品的δ 13 C值相比，溶解的苯和甲苯在向下梯度样品中的δ 13 C值富集了高达2+/-0.5 ‰的13 C。在向下梯度样品中溶解的苯和氘的δ 2 H值显示出相对于源区更大的同位素富集，苯高达27+/-5 ‰，氘高达50+/-5 ‰。观察到的碳和氢同位素分馏在downgradient样品提供的证据，在研究领域内的陶氏比荷卢BV的苯和三氯甲烷的生物降解。每个样品的碳和氢同位素组成的苯的生物降解的估计程度是一致的，支持的结论是，生物降解是所观察到的碳和氢同位素值的差异的主要控制。结合碳和氢同位素分析提供了在两个不同的参数的基础上，在现场比较生物降解的能力，因此提供了一个更强有力的基础，石油烃污染物的生物降解的评估。
Compound-specific carbon and hydrogen isotope analysis was used to investigate biodegradation of benzene and ethylbenzene in contaminated groundwater at Dow Benelux BV industrial site. delta13C values for dissolved benzene and ethylbenzene in downgradient samples were enriched by up to 2+/-0.5 per thousand, in 13C, compared to the delta13C value of the source area samples. delta2H values for dissolved benzene and ethylbenzene in downgradient samples exhibited larger isotopic enrichments of up to 27+/-5 per thousand for benzene and up to 50+/-5 per thousand for ethylbenzene relative to the source area. The observed carbon and hydrogen isotopic fractionation in downgradient samples provides evidence of biodegradation of both benzene and ethylbenzene within the study area at Dow Benelux BV. The estimated extents of biodegradation of benzene derived from carbon and hydrogen isotopic compositions for each sample are in agreement, supporting the conclusion that biodegradation is the primary control on the observed differences in carbon and hydrogen isotope values. Combined carbon and hydrogen isotope analyses provides the ability to compare biodegradation in the field based on two different parameters, and hence provides a stronger basis for assessment of biodegradation of petroleum hydrocarbon contaminants.