Immunodetection of pentamer and modified C-reactive protein using surface plasmon resonance biosensing
Immunodetection of pentamer and modified C-reactive protein using surface plasmon resonance biosensing
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DOI：
10.1016/j.bios.2005.11.001
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发表时间：
2006-02-15
影响因子：
12.6
通讯作者：
Chen, SJ
中科院分区：
文献类型：
--
作者：
Hu, WP;Hsu, HY;Chen, SJ
在临床实践中，五聚体c反应蛋白（pCRP）的检测通常被用作患者发生心血管疾病（CVD）风险的预后指标。通过对pCRP进行结构修饰，产生在体内表现出不同生物活性的修饰型CRP （mCRP）。近年来，mCRP被认为是一种比pCRP更强的诱导剂，因此mCRP的测定已成为评估心血管疾病发生风险的重要指标。表面等离子体共振（SPR）生物传感技术可以提高pCRP或mCRP的检测精度和实时性。在本研究中，三种单克隆抗体（Mabs）， C8；8 d8。和9制备过程。固定在蛋白G层上，用于随后的CRP检测。实验结果表明，Mab C8与pCRP和mCRP均有反应，Mab 8D8与pCRP有反应，Mab 9C9与mCRP有反应。未观察到非特异性结合引起的假信号。当使用Mab C8检测pCRP时，SPR生物测定法提供了足够的灵敏度来评估患者是否有发生心血管疾病的风险。SPR生物传感为实时评估pCRP和mCRP水平提供了一种可行且准确的方法，因此在心肺复苏术的临床检查中具有相当大的益处。(c) 2005 Elsevier B.V.版权所有
In clinical practices, the examination of pentamer C-reactive protein (pCRP) is commonly used as a prognostic indicator of the risk of a patient developing cardiovascular disease (CVD). Structural modification of pCRP produces a modified CRP (mCRP) which exhibits different biological activities in the body. In recent years, mCRP has come to be regarded as a more powerful inducer than pCRP, and hence mCRP measurement has emerged as an important indicator for assessing the risk of developing CVD. The surface plasmon resonance (SPR) biosensing technique can be employed to increase the detection accuracy and real-time response when sensing pCRP or mCRP. In this study, three monoclonal antibodies (Mabs), C8. 8D8. and 9C9. are immobilized on a protein G layer for subsequent CRP detection. The experimental results reveal that the Mab C8 reacts with both pCRP and mCRP, the Mab 8D8 with pCRP, and the Mab 9C9 with mCRP. No false signals caused by non-specific binding are observed. When detecting pCRP using Mab C8, the SPR bioassay provides sufficient sensitivity to evaluate whether or not a patient is at risk of developing CVD. SPR biosensing provides a viable and accurate approach for the real-time evaluation of pCRP and mCRP levels, and is therefore of considerable benefit in clinical examinations of CPR. (c) 2005 Elsevier B.V. All rights reserved.