Synthesis of Luminescent Graphene Quantum Dots with High Quantum Yield and Their Toxicity Study.
Synthesis of Luminescent Graphene Quantum Dots with High Quantum Yield and Their Toxicity Study.
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DOI：
10.1371/journal.pone.0144906
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Xu S
中科院分区：
文献类型：
--
作者：
Jiang D;Chen Y;Li N;Li W;Wang Z;Zhu J;Zhang H;Liu B;Xu S
高荧光量子产率石墨烯量子点是新一代生物成像材料。本工作以氧化石墨烯为前驱体，通过改进的光-芬顿反应和随后的水热过程制备了发光GQD。所制备的GQD是尺寸在2.3~6.4 nm之间的纳米材料，在水中发出强烈的绿色发光。荧光量子产率高达24.6%(激发波长为340 nm)，在pH值为7时荧光最强。此外，低浓度(12.5，25μg/mL)的GQD对细胞的形态、活力、膜完整性、细胞内活性氧水平和死亡率的影响较小，体外成像显示GQD主要位于细胞质区域。更引人注目的是，斑马鱼胚胎与GQD共培养进行活体成像，心率测试结果表明，摄入少量GQD对斑马鱼的心血管影响不大。量子产率高、光致发光强的GQD具有良好的生物相容性，在细胞成像、生物标记等生物医学领域显示出良好的应用前景。
High fluorescence quantum yield graphene quantum dots (GQDs) have showed up as a new generation for bioimaging. In this work, luminescent GQDs were prepared by an ameliorative photo-Fenton reaction and a subsequent hydrothermal process using graphene oxide sheets as the precursor. The as-prepared GQDs were nanomaterials with size ranging from 2.3 to 6.4 nm and emitted intense green luminescence in water. The fluorescence quantum yield was as high as 24.6% (excited at 340 nm) and the fluorescence was strongest at pH 7. Moreover, the influences of low-concentration (12.5, 25 μg/mL) GQDs on the morphology, viability, membrane integrity, internal cellular reactive oxygen species level and mortality of HeLa cells were relatively weak, and the in vitro imaging demonstrated GQDs were mainly in the cytoplasm region. More strikingly, zebrafish embryos were co-cultured with GQDs for in vivo imaging, and the results of heart rate test showed the intake of small amounts of GQDs brought little harm to the cardiovascular of zebrafish. GQDs with high quantum yield and strong photoluminescence show good biocompatibility, thus they show good promising for cell imaging, biolabeling and other biomedical applications.