Microfluidic device for concentration and SERS‐based detection of bacteria in drinking water
Microfluidic device for concentration and SERS‐based detection of bacteria in drinking water
复制标题
饮用水中细菌浓度及SERS检测的微流控装置
DOI：
10.1002/elps.202000048
复制
发表时间：
2020-05
期刊：
影响因子：
2.9
通讯作者：
Benjamin Krafft;Anna Týcová;Raphael D Urban;Christian Dusny;D. Belder
中科院分区：
文献类型：
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作者：
Benjamin Krafft;Anna Týcová;Raphael D Urban;Christian Dusny;D. Belder
不断需要开发易于操作的系统，以便快速和明确地识别饮用水中的细菌病原体，而不需要耗时的培养过程。在这项研究中，我们提出了一种一次性和低成本的芯片上实验室设备，利用纳米多孔膜连接两个堆叠的垂直微流控通道。其中一个通道提供样品，第二个通道通过潜在驱动力吸引样品。表面增强拉曼光谱(SERS)是一种可靠的细菌鉴定检测方法。为了获得表面增强的效果，在样品中加入了银纳米颗粒。膜的孔起到过滤器的作用，捕获微生物的身体以及纳米颗粒簇，为灵敏的SERS检测创造了合适的条件。其中，我们重点研究了器件的结构和性能表征。为了验证微流控芯片的功能，我们使用优化的实验参数分析了自来水中常见的病原菌(大肠杆菌DH5VLB120和黄山假单胞菌VLB120)。所获得的结果证实了我们的系统有希望构建一个一次性的光学平台，用于可靠和快速的病原体检测，将它们在集成的纳米孔膜上的电动浓度与SERS检测相结合。
There is a constant need for the development of easy‐to‐operate systems for the rapid and unambiguous identification of bacterial pathogens in drinking water without the requirement for time‐consuming culture processes. In this study, we present a disposable and low‐cost lab‐on‐a‐chip device utilizing a nanoporous membrane, which connects two stacked perpendicular microfluidic channels. Whereas one of the channels supplies the sample, the second one attracts it by potential‐driven forces. Surface‐enhanced Raman spectrometry (SERS) is employed as a reliable detection method for bacteria identification. To gain the effect of surface enhancement, silver nanoparticles were added to the sample. The pores of the membrane act as a filter trapping the bodies of microorganisms as well as clusters of nanoparticles creating suitable conditions for sensitive SERS detection. Therein, we focused on the construction and characterization of the device performance. To demonstrate the functionality of the microfluidic chip, we analyzed common pathogens (Escherichia coli DH5α and Pseudomonas taiwanensis VLB120) from spiked tap water using the optimized experimental parameters. The obtained results confirmed our system to be promising for the construction of a disposable optical platform for reliable and rapid pathogen detection which couples their electrokinetic concentration on the integrated nanoporous membrane with SERS detection.