Deciphering the roles of cell shape and Fat and Dachsous planar polarity in arranging the Drosophila apical microtubule network through quantitative image analysis.
Deciphering the roles of cell shape and Fat and Dachsous planar polarity in arranging the Drosophila apical microtubule network through quantitative image analysis.
复制标题
DOI：
10.1091/mbc.e22-09-0442
复制
发表时间：
2023-05-15
影响因子：
3.3
通讯作者：
Bulgakova, Natalia A
中科院分区：
文献类型：
--
作者：
Ramirez-Moreno, Miguel;Hunton, Robert;Strutt, David;Bulgakova, Natalia A
在上皮细胞中，平面极化的亚顶端微管网络被认为是重要的破坏细胞的对称性和维持所产生的细胞极性。对果蝇蛹翅和其他组织的研究表明，有两种不同的机制来确定网络极性。一方面，机械应变和/或细胞形状已被牵连作为关键的决定因素;另一方面，脂肪Dachsous平面极性途径已被认为是主要的极化线索。使用定量图像分析的蛹翼，我们重新评估这些模型。我们发现，细胞形状是一个强有力的预测微管组织在发展中的翅膀上皮。相反，脂肪Dachsous极性线索不发挥任何直接的作用，在组织的亚顶端微管网络，尽管能够弱招募微管负端帽蛋白Patronin细胞边界。我们的结论是，任何影响的脂肪Dachsous微管极性可能是间接的，通过其已知的能力，调节细胞的形状。
In epithelial cells, planar polarization of subapical microtubule networks is thought to be important for both breaking cellular symmetry and maintaining the resulting cellular polarity. Studies in the Drosophila pupal wing and other tissues have suggested two alternative mechanisms for specifying network polarity. On one hand, mechanical strain and/or cell shape have been implicated as key determinants; on the other hand, the Fat-Dachsous planar polarity pathway has been suggested to be the primary polarizing cue. Using quantitative image analysis in the pupal wing, we reassess these models. We found that cell shape was a strong predictor of microtubule organization in the developing wing epithelium. Conversely, Fat-Dachsous polarity cues do not play any direct role in the organization of the subapical microtubule network, despite being able to weakly recruit the microtubule minus-end capping protein Patronin to cell boundaries. We conclude that any effect of Fat-Dachsous on microtubule polarity is likely to be indirect, via their known ability to regulate cell shape.