CATECHOLAMINES IN FETAL AND NEWBORN RAT-BRAIN
CATECHOLAMINES IN FETAL AND NEWBORN RAT-BRAIN
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DOI：
10.1111/j.1471-4159.1973.tb04225.x
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发表时间：
1973-01-01
影响因子：
4.7
通讯作者：
HENRY, D
中科院分区：
文献类型：
--
作者：
COYLE, JT;HENRY, D
通过灵敏的放射性酶测定法测定胎鼠和新生大鼠脑中多巴胺和去甲肾上腺素的水平。在存在儿茶酚-O-甲基转移酶（EC 2.1.1.1）和[3 H-甲基]S-腺苷甲硫氨酸的情况下，将儿茶酚胺转化为其3-O-甲基化衍生物;并通过选择性提取分离[3 H]-衍生物。该测定法对多巴胺和去甲肾上腺素的灵敏度为100皮克，对至少30纳克的儿茶酚胺呈线性。这两种胺在妊娠15天时存在，在妊娠最后一周的含量增加了15倍。这些神经递质在新生大鼠脑中的区域分布与其生物合成酶的分布相关。利血平、非尼哌嗪、α-甲基帕拉酪氨酸、二乙基二硫代氨基甲酸酯和左旋多巴对18天妊娠胎仔脑中多巴胺和去甲肾上腺素水平影响的研究表明，这些神经递质的水平处于与已知成年大鼠脑中发生的水平相似的控制下。
The levels of dopamine and norepinephrine were determined in the brains of fetal and newborn rats by means of a sensitive, radiometric‐enzymatic assay. Catecholamines were converted to their 3‐O‐methylated derivatives in the presence of catechol‐O‐methyl transferase (EC 2.1.1.1) and [3H‐methyl]S‐adenosylmethionine; and the [3H]‐derivatives were isolated by selective extraction. The assay had a sensitivity for dopamine and norepinephrine of 100 picograms and was linear to at least 30 nanograms of catecholamines. Both amines were present at 15 days of gestation and increased 15‐fold in content during the last week of gestation. The regional distribution of these neurotransmitters in the brain of the newborn rat correlated with the distribution of their biosynthetic enzymes. An investigation of the effects of reserpine, pheniprazine, α‐methyl‐para‐tyrosine, diethyldithiocarbamate andl‐DOPA on the levels of dopamine and norepinephrine in the brains of the 18‐day gestational fetus indicated that the levels of these neurotransmitters are under controls similar to those known to occur in the brain of the adult rat.