Effect of albumin and fibrinogen on calcium phosphate formation on sol-gel-derived titania coatings in vitro
Effect of albumin and fibrinogen on calcium phosphate formation on sol-gel-derived titania coatings in vitro
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DOI：
10.1021/cm0107272
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发表时间：
2002-04-01
影响因子：
8.6
通讯作者：
Rosenholm, JB
中科院分区：
文献类型：
--
作者：
Areva, S;Peltola, T;Rosenholm, JB
溶胶-凝胶技术提供了一种制备多孔二氧化钛(TiO2)涂层的方法，这种涂层在体外可以诱导其表面形成类骨的羟基磷灰石。本研究在模拟体液中，研究了溶胶-凝胶法制备的二氧化钛涂层在有无白蛋白(BSA)和纤维蛋白原(Fib)存在的情况下，在体外形成磷酸钙的情况，以及表面固定蛋白对生物矿化过程的影响。通过离子浓度分析、X射线衍射仪、扫描电子显微镜、X射线光电子能谱等手段研究了蛋白质对磷酸钙形成的影响。当溶液中存在BSA和Fib时，CP层的生长动力学被强烈地抑制。结果表明，在溶胶-凝胶法制备的二氧化钛涂层上，类骨磷灰石的形成是通过连续的溶解/再沉淀过程进行的，在这个过程中，最初形成的CP相(S)重结晶为热力学上更稳定的相(S)，这与以前在其他生物材料中观察到的情况一样。在XPS实验中观察到了不均匀的电荷，这可以用来区分非晶态CP层和结晶度较差的CP区。
The sol-gel technique provides a method to produce porous titania (TiO2) coatings, which are known to induce bone-like hydroxyapatite formation on their surface in vitro. In this study, the calcium phosphate formation (in vitro bioactivity) on a sol-gel-derived titania coating was investigated in vitro in a simulated body fluid in the presence and absence of albumin (BSA) and fibrinogen (Fib) in solution as well as the effect of surface immobilized proteins on the biomineralization process. The effect of proteins on calcium phosphate (CP) formation was followed by ion concentration analysis, XRD, SEM-EDX, and XPS. When BSA and Fib were present in solution, the CP layer growth kinetics were strongly retarded. It is suggested that the bone-like apatite formation on sol-gel-derived titania coatings occurs via continuous dissolution/reprecipitation processes, where the initially formed CP phase(s) recrystallizes into a more thermodynamically stable phase(s), as previously observed for other biomaterials. Inhomogeneous charging was observed in the XPS experiments, which could be used to distinguish between an amorphous CP layer and poorly crystalline CP regions.