DYNAMIC EXPRESSION OF THE ONECUT TRANSCRIPTION FACTORS HNF-6, OC-2 AND OC-3 DURING SPINAL MOTOR NEURON DEVELOPMENT
DYNAMIC EXPRESSION OF THE ONECUT TRANSCRIPTION FACTORS HNF-6, OC-2 AND OC-3 DURING SPINAL MOTOR NEURON DEVELOPMENT
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DOI：
10.1016/j.neuroscience.2009.09.076
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发表时间：
2010-01-13
期刊：
影响因子：
3.3
通讯作者：
Clotman, F.
中科院分区：
文献类型：
--
作者：
Francius, C.;Clotman, F.
Onecut（OC）转录因子，即肝细胞核因子6（HNF-6）、OC-2和OC-3，是发育过程中表达于肝脏、胰腺和神经系统的转录激活因子。虽然它们在内胚层来源的组织和三叉神经节中的表达和作用已被研究，但它们在CNS中的表达尚未被描述。在这项研究中，我们报告了OC因子在发育中的脊髓运动神经元（MN）的定性和定量表达谱。我们提供的证据表明，OC的表达是在新生儿MN启动。在后期阶段，它们在四个运动列内的分化运动神经元的子集中差异地和动态地表达。我们还发现，脊髓MN中HNF-6的表达谱在鸡胚中是保守的。总之，我们的数据揭示了脊髓MN中OC蛋白的复杂和动态表达谱，这表明这些因子可能参与控制运动神经元发育不同步骤的调控网络。(C)2010年IBRO。由爱思唯尔有限公司出版。保留所有权利。
The Onecut (OC) transcription factors, namely Hepatocyte nuclear factor 6 (HNF-6), OC-2 and OC-3, are transcriptional activators expressed in liver, pancreas and nervous system during development. Although their expression and roles in endoderm-derived tissues and in the trigeminal ganglia have been investigated, their expression in the CNS has not been described yet. In this study, we report a qualitative and quantitative expression profile of the OC factors in the developing spinal motor neurons (MN). We provide evidence that OC expression is initiated in newly-born MN. At later stages, they are differentially and dynamically expressed in subsets of differentiating motor neuron within the four motor columns. We also show that the expression profile of HNF-6 in spinal MN is conserved in chick embryos. Together, our data unveil a complex and dynamic expression profile of the OC proteins in spinal MN, which suggests that these factors may participate in regulatory networks that control different steps of motor neuron development. (C) 2010 IBRO. Published by Elsevier Ltd. All rights reserved.