Selection of a DNA aptamer for the development of fluorescent aptasensor for carbaryl detection
Selection of a DNA aptamer for the development of fluorescent aptasensor for carbaryl detection
复制标题
选择 DNA 适体来开发用于西维因检测的荧光适体传感器
DOI：
10.1016/j.cclet.2021.01.016
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发表时间：
2021-06-05
影响因子：
9.1
通讯作者：
He, Nongyue
中科院分区：
文献类型：
--
作者：
Liu, Yuan;Yang, Gaojian;He, Nongyue
应用改进的ssDNA文库固定化富集配体系统进化（SELEX）技术筛选西维因的适体。在9轮选择后，获得高度富集的ssDNA池。Apta 3被证明是最佳适体。为了便于适配体的修饰，Apta 3被进一步截短，解离常数（Kd）为0.364 +/- 0.055 μ mol/L，并开发了荧光适配体传感器。西维因的线性范围为100 ~ 1500 nmol/L，检出限为15.23 nmol/L。对西维因加标真实的样品进行了验证，回收率在97.7%~ 107.3%之间。(C)2021中国化学会、中国医学科学院药物研究所。Elsevier B. V.出版，保留所有权利。
An improved ssDNA library immobilized systematic evolution of ligands by enrichment (SELEX) was applied to select aptamers against carbaryl. After nine selection rounds, a highly enriched ssDNA pool was obtained. The Apta3 was demonstrated as the optimal aptamer. In order to facilitate the modification of aptamer, the Apta3 was further truncated with the dissociation constant (K-d) of 0.364 +/- 0.055 mu mol/L and a fluorescent aptasensor was developed. The linear range for carbaryl was from 100 nmol/L to 1500 nmol/L, with the limit of detection was as low as 15.23 nmol/L. Besides, the biosensor was validated for the carbaryl spiked real samples, and the recoveries were between 97.7% and 107.3%. (C) 2021 Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. Published by Elsevier B.V. All rights reserved.