Designer short peptide surfactants stabilize G protein-coupled receptor bovine rhodopsin
Designer short peptide surfactants stabilize G protein-coupled receptor bovine rhodopsin
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DOI：
10.1073/pnas.0607167103
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发表时间：
2006-11
期刊：
影响因子：
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通讯作者：
Xiaojun Zhao;Y. Nagai;P. Reeves;Patrick J. Kiley;H. Khorana;Shuguang Zhang
中科院分区：
文献类型：
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作者：
Xiaojun Zhao;Y. Nagai;P. Reeves;Patrick J. Kiley;H. Khorana;Shuguang Zhang
膜蛋白在细胞活动的各个方面都发挥着至关重要的作用。为了研究不同的膜蛋白，选择合适的表面活性剂来稳定它们以进行分析至关重要。尽管付出了很多努力，但在阐明其结构和功能方面进展甚微，这主要是由于缺乏合适的表面活性剂。在这里，我们报告了使用一类新型设计的短肽表面活性剂来稳定溶液中的 G 蛋白偶联受体牛视紫红质。这些表面活性剂由七种氨基酸组成，具有亲水性头部（天冬氨酸或赖氨酸）和具有六个连续丙氨酸的疏水性尾部。这些肽表面活性剂不仅增强了牛视紫红质在脂质和常见表面活性剂正十二烷基-β-d-麦芽糖苷和辛基-d-葡萄糖苷存在下的稳定性，而且它们还在热变性条件下显着稳定视紫红质，即使在去除脂质后也是如此。这些肽表面活性剂简单、通用、有效且价格实惠。它们代表了一种设计分子纳米材料，可用于研究各种难以捉摸的膜蛋白。
Membrane proteins play vital roles in every aspect of cellular activities. To study diverse membrane proteins, it is crucial to select the right surfactants to stabilize them for analysis. Despite much effort, little progress has been made in elucidating their structure and function, largely because of a lack of suitable surfactants. Here we report the stabilization of a G protein-coupled receptor bovine rhodopsin in solution, using a new class of designer short and simple peptide surfactants. These surfactants consist of seven amino acids with a hydrophilic head, aspartic acid or lysine, and a hydrophobic tail with six consecutive alanines. These peptide surfactants not only enhance the stability of bovine rhodopsin in the presence of lipids and the common surfactants n-dodecyl-β-d-maltoside and octyl-d-glucoside, but they also significantly stabilize rhodopsin under thermal denaturation conditions, even after lipids are removed. These peptide surfactants are simple, versatile, effective, and affordable. They represent a designer molecular nanomaterial for use in studies of diverse elusive membrane proteins.