Cyclin B1 mRNA translation is temporally controlled through formation and disassembly of RNA granules.
Cyclin B1 mRNA translation is temporally controlled through formation and disassembly of RNA granules.
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DOI：
10.1083/jcb.201302139
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发表时间：
2013-09-30
期刊：
影响因子：
--
通讯作者：
Yamashita M
中科院分区：
文献类型：
--
作者：
Kotani T;Yasuda K;Ota R;Yamashita M
含有Cyclin B1转录本的RNA颗粒的时间调控形成对于精确的翻译激活时间至关重要。信使RNA翻译的时间控制是调节细胞、神经元和发育过程的重要机制。然而，协调翻译激活时间的机制在很大程度上仍然没有解决。成熟的卵母细胞在前期I停止减数分裂，并沉积休眠的mRNAs。其中，成熟诱导激素对细胞周期蛋白B1mRNA的翻译控制对卵母细胞成熟的正常进行非常重要，卵母细胞通过这一过程获得生育能力。在本研究中，我们发现在斑马鱼和小鼠卵母细胞中，处于休眠状态的细胞周期蛋白B1在细胞质中形成颗粒。翻译的实时成像显示，颗粒在成熟过程中翻译激活时解体。细胞周期蛋白B1RNA颗粒的形成需要将其与Pumilio1蛋白结合，并依赖于肌动蛋白细丝。Cyclin B1 RNA颗粒的破坏加速了其成熟后翻译激活的时间，而稳定则阻碍了翻译激活。因此，我们的结果表明，RNA颗粒的形成对于调节翻译激活的时间至关重要。
Temporally regulated formation of RNA granules containing cyclin B1 transcript is critical for the precise timing of translational activation. Temporal control of messenger RNA (mRNA) translation is an important mechanism for regulating cellular, neuronal, and developmental processes. However, mechanisms that coordinate timing of translational activation remain largely unresolved. Full-grown oocytes arrest meiosis at prophase I and deposit dormant mRNAs. Of these, translational control of cyclin B1 mRNA in response to maturation-inducing hormone is important for normal progression of oocyte maturation, through which oocytes acquire fertility. In this study, we found that dormant cyclin B1 mRNA forms granules in the cytoplasm of zebrafish and mouse oocytes. Real-time imaging of translation revealed that the granules disassemble at the time of translational activation during maturation. Formation of cyclin B1 RNA granules requires binding of the mRNA to Pumilio1 protein and depends on actin filaments. Disruption of cyclin B1 RNA granules accelerated the timing of their translational activation after induction of maturation, whereas stabilization hindered translational activation. Thus, our results suggest that RNA granule formation is critical for the regulation of timing of translational activation.
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影响因子：
11.4
作者：
de Moor, CH;Richter, JD
通讯作者：
Richter, JD
影响因子：
2.7
作者：
KATSU, Y;YAMASHITA, M;NAGAHAMA, Y
通讯作者：
NAGAHAMA, Y
影响因子：
2.7
作者：
Kloc, M;Dougherty, MT;Etkin, LD
通讯作者：
Etkin, LD
mRNA 脱帽和 P 体形成的控制。
影响因子：
16
作者：
Franks, Tobias M.;Lykke-Andersen, Jens
通讯作者：
Lykke-Andersen, Jens
DOI：
10.1073/pnas.93.25.14602
发表时间：
1996-12-10
影响因子：
11.1
作者：
Gebauer, F;Richter, JD
通讯作者：
Richter, JD