Plant PIEZO homologs modulate vacuole morphology during tip growth
Plant PIEZO homologs modulate vacuole morphology during tip growth
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DOI：
10.1126/science.abe6310
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发表时间：
2021-07-30
期刊：
影响因子：
56.9
通讯作者：
Haswell, Elizabeth S.
中科院分区：
文献类型：
--
作者：
Radin, Ivan;Richardson, Ryan A.;Haswell, Elizabeth S.
在动物中，PIEZO 是质膜定位的阳离子通道，参与多种机械感觉过程。我们研究了苔藓小立碗藓和开花植物拟南芥尖端生长细胞的 PIEZO 功能。 PpPIEZO1 和 PpPIEZO2 对茎细胞的正常生长、大小和细胞质钙振荡有过多的贡献。 PpPIEZO1 和 PpPIEZO2 均定位于液泡膜。功能丧失、功能获得和过度表达突变体表明，苔藓 PIEZO 同源物通过管状、内化和/或裂变促进液泡膜复杂性的增加。拟南芥 PIEZO1 也定位于液泡膜，并且是花粉管尖端的液泡管所必需的。我们认为，在植物细胞中，液泡膜比质膜具有更大的运动自由度，使其成为机械感觉蛋白更有效的位置。
In animals, PIEZOs are plasma membrane-localized cation channels involved in diverse mechanosensory processes. We investigated PIEZO function in tip-growing cells in the moss Physcomitrium patens and the flowering plant Arabidopsis thaliana. PpPIEZO1 and PpPIEZO2 redundantly contribute to the normal growth, size, and cytoplasmic calcium oscillations of caulonemal cells. Both PpPIEZO1 and PpPIEZO2 localized to vacuolar membranes. Loss-of-function, gain-of-function, and overexpression mutants revealed that moss PIEZO homologs promote increased complexity of vacuolar membranes through tubulation, internalization, and/or fission. Arabidopsis PIEZO1 also localized to the tonoplast and is required for vacuole tubulation in the tips of pollen tubes. We propose that in plant cells the tonoplast has more freedom of movement than the plasma membrane, making it a more effective location for mechanosensory proteins.