Electrophoresis of human red blood cells and platelets. Evidence for depletion of dextran
Electrophoresis of human red blood cells and platelets. Evidence for depletion of dextran
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DOI：
10.1016/0006-355x(96)00026-1
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发表时间：
1996-07-01
期刊：
影响因子：
1.1
通讯作者：
Kiesewetter, H
中科院分区：
文献类型：
--
作者：
Baumler, H;Donath, E;Kiesewetter, H
本文提出了光滑表面和毛状表面附近聚合物耗尽层的理论描述，并用于解释实验。在葡聚糖（M（W）= 70 kD）存在下，对人红细胞和血小板在电解质水溶液中的电泳迁移率进行了测量，以研究葡聚糖与细胞表面的相互作用。在葡聚糖存在下的电泳迁移率比从粘度预期的要大得多。这种行为被解释为存在聚合物耗尽层的证据。根据离子强度，发现耗尽层厚度范围为2.9至4.4 nm。糖萼外边界处的葡聚糖浓度仅为体积值的10%，不能排除这种少量反映吸附的可能性。在血小板的情况下，血小板表面的葡聚糖消耗量随离子强度的降低而明显减小，表明葡聚糖与血小板表面的相互作用比与红细胞表面的相互作用更为复杂。得出的结论是，离子强度依赖性渗透的葡聚糖进入毛层比离子强度依赖性吸附的变化更可能，如在红细胞的情况下，可能存在一些葡聚糖的吸附。版权所有（C）1996 Elsevier Science Ltd
A theoretical description of polymer depletion layers near smooth and hairy surfaces is developed and used for interpretation of experiments, Electrophoretic mobility measurements of human red blood cells and platelets in aqueous electrolyte solutions were performed in the presence of dextran (M(W) = 70 kD) to study the interaction of dextran with the cell surface. The electrophoretic mobility in the presence of dextran was considerably larger than expected from the viscosity. This behavior was interpreted as evidence for the existence of a polymer depletion layer, Depending on ionic strength, depletion layer thicknesses ranging from 2.9 to 4.4 nm were found, The dextran concentration at the outer border of the glycocalyx was only 10% of the bulk value, One cannot exclude the possibility that this small amount reflected adsorption, In the case of platelets, the degree of the mobility reduction depended on ionic strength, Depletion of dextran from the platelet surface apparently became smaller with decreasing ionic strength, This indicated a more complex pattern of interaction of dextran with the platelet surface than with the RBC surface, Both adsorption and polymer penetration into the glycocalyx were discussed quantitatively. It was concluded that ionic strength-dependent penetration of dextran into the hairy layer is more likely than ionic strength dependent adsorption changes, As in the case of red cells, some adsorption of dextran might be present. Copyright (C) 1996 Elsevier Science Ltd