[An ultrastructural study of capillary permeability of rabbit ovarian follicles during ovulation using carbon tracer (author's transl)].
[An ultrastructural study of capillary permeability of rabbit ovarian follicles during ovulation using carbon tracer (author's transl)].
复制标题
[利用碳示踪剂对兔排卵期间毛细血管通透性进行超微结构研究（作者译）]。
DOI：
--
复制
发表时间：
1980
期刊：
影响因子：
--
通讯作者：
T. Nishimura
中科院分区：
文献类型：
--
作者：
Y. Okuda;H. Okamura;H. Kanzaki;A. Takenaka;K. Morimoto;T. Nishimura
观察到卵巢的卵泡周围毛细血管在排卵时变得充血，内膜和外膜明显水肿，提示排卵前卵泡周围毛细血管的通透性增加。在以往的研究中，我们报道了排卵前卵泡周围毛细血管通透性增加的超微结构证据。本研究进一步用碳粒示踪法观察了家兔注射hCG诱导排卵后不同时间卵泡周围毛细血管的变化。在排卵前各阶段，胞饮小泡的大小、数量和分布均无显著变化。碳颗粒没有被吸收进去。注射hCG后0、3、4、6小时观察到穿孔，4小时最多，但没有碳颗粒能穿透它们。注射hCG后10小时，两个相邻的内皮细胞之间开始观察到细胞间隙。碳颗粒穿过这些间隙，留在基底膜内（所谓的壁内泄漏）。这些观察结果表明，排卵前卵泡周围毛细血管的通透性增加是由于细胞间的间隙。
The observations that perifollicular capillaries of the ovary become engorged with prominent edema in the theca interna and externa around the time of ovulation, suggest a preovulatory increase in permeability of perifollicular capillaries. In previous study, we reported ultrastructural evidences which suggested the increased permeability of perifollicular capillaries just prior to ovulation. In this study, we further examined the perifollicular capillaries of rabbit ovary by using carbon particles as a tracer at various time intervals after the hCG injection for induction of ovulation. Pinocytotic vesicles exhibited no significant changes in size, number and distribution through all preovulatory stages. Carbon particles were not taken into them. Fenestrations were observed at 0,3,4,6 hrs after the hCG injection, most frequently at 4 hrs, but no carbon particles could penetrate them. Intercellular gaps between two adjacent endothelial cells began to be observed at 10 hrs after the hCG injection. Carbon particles passed through these gaps with staying inside the basement membrane (so-called intramural leaking). These observations indicated that the increased permeability of the perifollicular capillaries, just before ovulation, were due to intercellular gaps.