Porcine sinus mucosa holds cells that respond to bone morphogenetic protein (BMP)-6 and BMP-7 with increased osteogenic differentiation in vitro
Porcine sinus mucosa holds cells that respond to bone morphogenetic protein (BMP)-6 and BMP-7 with increased osteogenic differentiation in vitro
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DOI：
10.1111/j.1600-0501.2004.01062.x
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发表时间：
2004-10-01
影响因子：
4.3
通讯作者：
Watzek, G
中科院分区：
文献类型：
--
作者：
Gruber, R;Kandler, B;Watzek, G
这项体外研究的目的是确定窦粘膜是否含有具有成骨潜力的细胞。研究了来自三只成年猪的窦粘膜的冷冻切片的STRO-1（间充质祖细胞的标志物）和碱性磷酸酶活性（由定向成骨谱系的细胞和成熟成骨细胞表达的酶）的表达。为了确定它们的成骨潜能，将粘膜来源的细胞与骨形态发生蛋白（BMP）-6和BMP-7孵育，并测量碱性磷酸酶活性、骨钙素表达和细胞外基质的矿化。我们发现窦粘膜细胞STRO-1染色阳性和碱性磷酸酶活性。当窦粘膜组织被置于培养物中时，碱性磷酸酶阳性细胞从外植体中生长出来，并进一步增加碱性磷酸酶活性以响应BMP-6和BMP-7。成骨细胞特异性细胞外基质蛋白骨钙素的表达水平和细胞外基质内钙积累的量也响应于BMP而增加。我们的结论是窦粘膜持有间充质祖细胞和细胞致力于成骨谱系，可以响应BMP-6和BMP-7的成骨分化的增加。
The aim of this in vitro study was to determine whether the sinus mucosa holds cells with an osteogenic potential. Frozen sections of sinus mucosa from three adult pigs were investigated for the expression of STRO-1, a marker of mesenchymal progenitor cells, and alkaline phosphatase activity, an enzyme expressed by cells committed to the osteogenic lineage and by mature osteoblasts. To determine their osteogenic potential, mucosa-derived cells were incubated with bone morphogenetic protein (BMP)-6 and BMP-7, and alkaline phosphatase activity, osteocalcin expression, and mineralization of the extracellular matrix was measured. We found sinus mucosa cells staining positive for STRO-1 and alkaline phosphatase activity. When sinus mucosa tissue was placed in culture, alkaline phosphatase positive cells grew out from the explants and further increased alkaline phosphatase activity in response to BMP-6 and BMP-7. The expression level of the osteoblast-specific extracellular matrix protein osteocalcin, and the amount of calcium accumulation within the extracellular matrix was also increased in response to BMPs. We conclude that the sinus mucosa holds mesenchymal progenitor cells and cells committed to the osteogenic lineage that can respond to BMP-6 and BMP-7 by an increase of their osteogenic differentiation.