IDENTIFICATION AND CHARACTERIZATION OF THE EGR-1 GENE-PRODUCT, A DNA-BINDING ZINC FINGER PROTEIN-INDUCED BY DIFFERENTIATION AND GROWTH SIGNALS
IDENTIFICATION AND CHARACTERIZATION OF THE EGR-1 GENE-PRODUCT, A DNA-BINDING ZINC FINGER PROTEIN-INDUCED BY DIFFERENTIATION AND GROWTH SIGNALS
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DOI：
10.1128/mcb.10.5.1931
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发表时间：
1990-05-01
影响因子：
5.3
通讯作者：
SUKHATME, VP
中科院分区：
文献类型：
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作者：
CAO, XM;KOSKI, RA;SUKHATME, VP
Egr-1是一种由多种信号诱导的立即早期反应基因，启动生长和分化。它的cDNA序列预测了一种具有锌指的蛋白质。我们已经产生了Egr-1基因产物的抗血清，并将其鉴定为血清刺激的小鼠成纤维细胞中的80千道尔顿短寿命蛋白。大鼠Egr-1产物也已在神经生长因子诱导的PC 12细胞中鉴定。此外，我们通过细胞分级分离和免疫细胞化学表明，Egr-1蛋白位于细胞核中。我们还证明它是磷酸化的。体外产生的Egr-1蛋白以锌依赖性方式与序列CGCCCCCGC高亲和力结合。
Egr-1 is an immediate-early response gene induced by diverse signals that initiate growth and differentiation. Its cDNA sequence predicts a protein with zinc fingers. We have generated an antiserum to the Egr-1 gene product and identified it as an 80-kilodalton short-lived protein in serum-stimulated mouse fibroblasts. The rat Egr-1 product has also been identified in nerve growth factor-induced PC12 cells. In addition, we show by cell fractionation and immunocytochemistry that the Egr-1 protein is located in the nucleus. We also demonstrate that it is phosphorylated. In vitro-generated Egr-1 protein binds with high affinity to the sequence CGCCCCCGC in a zinc-dependent manner.