Recognition of dengue virus NS1-NS2a proteins by human CD4+ cytotoxic T lymphocyte clones
Recognition of dengue virus NS1-NS2a proteins by human CD4+ cytotoxic T lymphocyte clones
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DOI：
10.1006/viro.1997.8648
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发表时间：
1997-08-04
期刊：
影响因子：
3.7
通讯作者：
Ennis, FA
中科院分区：
文献类型：
--
作者：
Green, S;Kurane, I;Ennis, FA
我们利用含有登革病毒蛋白编码基因的重组痘苗病毒，检测了9个登革病毒特异性人CD4+ CD8-细胞毒性T淋巴细胞（CTL）克隆的蛋白识别。这些克隆是从先前接种过实验性登革热减毒活疫苗的供者的外周血单个核细胞中建立的。在9个CD4+ T细胞克隆中，7个是登革热-1特异性的，2个是登革热-1- 3交叉反应性的。4个登革热-1特异性克隆和1个登革热-1-登革热-3交叉反应克隆识别出NS1或NS2a蛋白内的表位。人白细胞抗原（HLA）限制性分析显示，3个登革热-1特异性克隆为HLA- dr1限制性克隆，1个登革热-1-登革热-3交叉反应性克隆为HLA- dpw3限制性克隆。这些结果表明，早期报道的NS1和NS2a蛋白以及C、E和NS3蛋白含有一个或多个能被登革热病毒特异性的人CD4+ T淋巴细胞识别的表位。(C) 1997学术出版社。
We examined nine dengue virus-specific human CD4+ CD8- cytotoxic T lymphocyte (CTL) clones for protein recognition, using recombinant vaccinia viruses which contain genes coding for dengue virus proteins. These clones were established from peripheral blood mononuclear cells of a donor previously immunized with a live-attenuated experimental dengue-l vaccine. Of nine CD4+ T cell clones, seven were dengue-l-specific and two were dengue-1-dengue-3 cross-reactive. Four dengue-1-specific clones and one dengue-1-dengue-3 cross-reactive clone recognized epitopes within the NS1 or NS2a proteins. Analysis of human leukocyte antigen (HLA) restriction revealed that three dengue-l-specific clones are HLA-DR1-restricted and one dengue-1-dengue-3 cross-reactive clone is HLA-DPw3-restricted. These results indicate that NS1 and NS2a proteins as well as C, E, and NS3 proteins reported earlier contain one or more epitopes recognized by dengue virus-specific human CD4+ T lymphocytes. (C) 1997 Academic Press.