Mediation of coffee-induced improvements in human vascular function by chlorogenic acids and its metabolites: Two randomized, controlled, crossover intervention trials
Mediation of coffee-induced improvements in human vascular function by chlorogenic acids and its metabolites: Two randomized, controlled, crossover intervention trials
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DOI：
10.1016/j.clnu.2016.11.013
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发表时间：
2017-12-01
影响因子：
6.3
通讯作者：
Spencer, Jeremy P. E.
中科院分区：
文献类型：
--
作者：
Mills, Charlotte E.;Flury, Andreas;Spencer, Jeremy P. E.
背景与目的：多酚的摄入与人体血管功能的改善有关，尽管有关羟基肉桂酸酯，如绿原酸(CGA)的数据尚未被研究。目的：研究饮用富含绿原酸的咖啡对人体血管功能的影响以及CGAs是否参与了潜在的影响。方法：采用随机、对照、交叉的两种急性人体干预试验。在15名健康男性受试者中，研究了与咖啡因相匹配但CGA含量不同(89和310毫克)的咖啡摄入量对血流介导性扩张(FMD)的影响。在对24名健康男性受试者进行的第二次干预试验中，纯5-咖啡酰奎宁酸(5-CQA)，咖啡中的主要CGA(5-CQA；结果：我们观察到摄入低和高多酚(mg和310 mg CGA)后的双相FMD反应，在饮用咖啡后1小时(分别为1.10+/-0.43%和1.34+/-0.62%)和5小时(分别为0.79%+/-0.32和1.52%+/-0.40)增加。对咖啡摄入的FMD反应与血浆中CGA代谢物的出现密切相关，特别是1h的3-、4-和5-阿魏酰奎宁酸和阿魏酸-4‘-O-硫酸盐，以及5h的异阿魏酸-3’-O-葡萄糖醛酸脂和阿魏酸-4‘-O-硫酸盐。给予纯化的5-CQA(450 Mg)也能改善FMD反应，与对照组相比(干预后1h为0.75+/-1.31%，p=0.06)，并伴随着血浆代谢物的出现。结论：摄入咖啡可显著改善人体血管功能，这在一定程度上是一种效果由5-CQA及其生理代谢产物介导。(C)2016年提交人(S)。爱思唯尔有限公司出版。
Background & aims: Polyphenol intake has been linked to improvements in human vascular function, although data on hydroxycinnamates, such as chlorogenic acid (CGA) have not yet been studied. We aimed to investigate the impact of coffee intake rich in chlorogenic acid on human vascular function and whether CGAs are involved in potential effects.Methods: Two acute randomized, controlled, cross-over human intervention trials were conducted. The impact of coffee intake, matched for caffeine but differing in CGA content (89, and 310 mg) on flow mediated dilatation (FMD) was assessed in 15 healthy male subjects. In a second intervention trial conducted with 24 healthy male subjects, the impact of pure 5-caffeoylquinic acid (5-CQA), the main CGA in coffee (5-CQA; 450 mg and 900 mg) on FMD was also investigated.Results: We observed a bi-phasic FMD response after low and high polyphenol, (89 mg and 310 mg CGA) intake, with increases at 1 (1.10 +/- 0.43% and 1.34 +/- 0.62%, respectively) and 5 (0.79% +/- 0.32 and 1.52% +/- 0.40, respectively) hours post coffee consumption. FMD responses to coffee intake was closely paralleled by the appearance of CGA metabolites in plasma, notably 3-, 4- and 5-feruloylquinic acid and ferulic-4'-O-sulfate at 1 h and isoferulic-3'-O-glucuronide and ferulic-4'-O-sulfate at 5 h. Intervention with purified 5-CQA (450 mg) also led to an improvement in FMD response relative to control (0.75 +/- 1.31% at 1 h post intervention, p = 0.06) and concomitant appearance of plasma metabolites.Conclusions: Coffee intake acutely improves human vascular function, an effect, in part, mediated by 5-CQA and its physiological metabolites. (C) 2016 The Author(s). Published by Elsevier Ltd.