CNTF promotes the regrowth or retinal ganglion cell axons into murine peripheral nerve grafts
CNTF promotes the regrowth or retinal ganglion cell axons into murine peripheral nerve grafts
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DOI：
10.1097/00001756-200012180-00019
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发表时间：
2000-12-18
期刊：
影响因子：
1.7
通讯作者：
Harvey, AR
中科院分区：
文献类型：
--
作者：
Cui, Q;Harvey, AR
将自体周围神经移植到成年小鼠的横切视神经上。我们检查了眼内注射 CNTF 是否会增加视网膜神经节细胞 (RGC) 轴突再生，以及哪些类型的 RGC 将轴突重新长成移植物。颞部 CNTF 眼部注射后，荧光金标记的再生 RGC（平均值 +/- s.e.m. 342 +/- 113.1；n = 6）比注射假眼的小鼠（133 +/- 27.6；n = 8）更多。在接受鼻腔和颞部 CNTF 注射的小鼠中观察到更多数量的再生 RGC（1198 +/- 367.6；n = 6）。再生视网膜和正常视网膜中的体细胞区域范围相似，但再生 RGC 的平均大小更大（212 mum(2) vs 111 mum(2)）。大多数再生的 RGC 都有大的树突区域。数据表明 RGC 优先将轴突再生为 PN 移植物。 NeuroReport 11：3999-4002 (C) 2000 Lippincott Williams & Wilkins。
Autologous peripheral nerves were transplanted onto transected optic nerves of adult mice. We examined whether intraocular CNTF injections increased retinal ganglion cell (RGC) axon regeneration, and what types of RGCs regrew axons into grafts. After temporal CNTF eye injections there were more fluorogold-labelled regenerating RGCs (mean +/- s.e.m. 342 +/- 113.1; n = 6) than in sham eye-injected mice (133 +/- 27.6; n = 8). Greater numbers of regenerating RGCs (1198 +/- 367.6; n = 6) were seen in mice receiving both nasal and temporal CNTF injections. The range of soma areas in regenerate and normal retinas was similar but the average size of regenerating RGCs was greater (212 mum(2) vs 111 mum(2)). Most regenerating RGCs had large dendritic fields. The data suggest RGCs preferentially regrowing axons into PN grafts. NeuroReport 11:3999-4002 (C) 2000 Lippincott Williams & Wilkins.