RAPID ISOELECTRIC-FOCUSING IN A VERTICAL POLYACRYLAMIDE MINIGEL SYSTEM
RAPID ISOELECTRIC-FOCUSING IN A VERTICAL POLYACRYLAMIDE MINIGEL SYSTEM
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DOI：
10.1016/0003-2697(87)90166-7
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发表时间：
1987-12-01
影响因子：
2.9
通讯作者：
REEVES, HC
中科院分区：
文献类型：
--
作者：
ROBERTSON, EF;DANNELLY, HK;REEVES, HC
描述了一种在垂直聚丙烯酰胺米尼格尔系统中采用等电聚焦的蛋白质快速分辨方法。等电聚焦可以在3小时内完成，利用低电压，在自然条件下或变性条件下，在8 M尿素的存在。该程序允许应用比其他等电聚焦程序含有更多蛋白质的更大样本量，并提供板凝胶比管凝胶分析目的的额外优势。该程序也适用于二维电泳技术，可以在1天内完成二维凝胶。
A rapid method is described for the resolution of proteins employing isoelectric focusing in a vertical polyacrylamide minigel system. Isoelectric focusing can be performed in only 3 h, utilizing low voltage, under either native conditions or denaturing conditions in the presence of 8 M urea. The procedure permits the application of larger sample volumes containing more protein than other isoelectric focusing procedures, and provides the additional advantages of slab gels over tube gels for analytical purposes. The procedure is also well adapted for use in two-dimensional electrophoretic techniques, making it possible to complete a two-dimensional gel in 1 day.