Prolonged Pain Research in Mice: Trends in Reference to the 3Rs
Prolonged Pain Research in Mice: Trends in Reference to the 3Rs
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DOI：
10.1080/10888705.2013.741004
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发表时间：
2013-01-01
影响因子：
1.5
通讯作者：
Briese, Lauren
中科院分区：
文献类型：
--
作者：
Balcombe, Jonathan;Ferdowsian, Hope;Briese, Lauren
这篇文献综述记录了在长期疼痛研究中使用小鼠的趋势，本文将其定义为让小鼠承受至少 14 天的疼痛源的研究。过去十年中，针对小鼠的长期疼痛研究总量急剧增加，针对所检查的 3 种疼痛类别：神经性疼痛、炎症性疼痛和慢性疼痛。长期小鼠疼痛研究的数量占所有小鼠研究和所有小鼠疼痛研究的比例也显着增加。在长期疼痛研究中转基因小鼠的使用也显着增加，尽管在长期疼痛研究中使用的所有小鼠中所占的比例并不高。对于 3 种疼痛类别中的任何一种或 3 种常见疼痛诱发模型中的任何一种：慢性压迫性损伤、部分坐骨神经结扎和完全弗氏佐剂，每项研究使用的小鼠数量总体上没有显着变化。最后，尽管大多数作者提到了非人类动物使用机构委员会对实验的批准，但在随机选择的 55 篇论文中没有提及 3R。鉴于侵入性研究中使用的小鼠数量比例较高，以及造成持久痛苦的研究的严重性，这些趋势引发了严重的问题：研究人员和机构是否正确实施了替代、减少和细化的 3R 原则。
This literature review documents trends in the use of mice in prolonged pain research, defined herein as research that subjects mice to a source of pain for at least 14 days. The total amount of prolonged pain research on mice has increased dramatically in the past decade for the 3 pain categories examined: neuropathic, inflammatory, and chronic pain. There has also been a significant rise in the number of prolonged mouse pain studies as a proportion of all mouse studies and of all mouse pain studies. The use of transgenic mice has also risen significantly in prolonged pain research, though not as a proportion of all mice used in prolonged pain research. There has not been significant overall change in the number of mice being used per study for any of the 3 pain categories or for any of 3 common pain inducement models: chronic constriction injury, partial sciatic nerve ligation, and complete Freund's adjuvant. Finally, although most authors referred to approval of experiments by an institutional nonhuman animal use committee, there were no references to the 3Rs in a random selection of 55 papers examined. Given the proportionally high volume of mice used in invasive research and the gravity of studies that inflict lasting pain, these trends raise serious questions about whether the 3Rs principles of Replacement, Reduction, and Refinement are being appropriately implemented by researchers and institutions.