Genes with expression levels correlating to drip loss prove association of their polymorphism with water holding capacity of pork
Genes with expression levels correlating to drip loss prove association of their polymorphism with water holding capacity of pork
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DOI：
10.1007/s11033-011-0714-5
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发表时间：
2012-01-01
影响因子：
2.8
通讯作者：
Ponsuksili, S.
中科院分区：
文献类型：
--
作者：
Brunner, R. M.;Srikanchai, T.;Ponsuksili, S.
已知表现出与滴水损失BVES、SLC 3A 2、ZDHHC 5、CS、COQ 9和EGFR相关的表达水平的六个基因已用于候选基因分析。基于计算机模拟分析，检测SNP，通过测序确认，并用于基因分型。对来自6个猪群的约1，800头动物进行了SNP基因分型，这些猪群包括商业猪群皮特兰（PI）和德国长白猪（DL）、不同商业猪群皮特兰×（德国大白色×德国长白猪）（PIF 1（a/B/c））和一个实验F-2群杜洛克×皮特兰（DUPI）。比较和遗传作图分别确定了BVES在SSC 1上、SLC 3A 2和ZDHHC 5在SSC 2上、CS在SSC 5上、COQ 9在SSC 6上、EGFR在SSC 9上的定位，与胴体和肉质性状的QTL区域相吻合。BVES，SLC 3A 2，和CS显示关联至少与滴水损失和其他几个措施的持水量（WHC）。此外，COQ 9和EGFR与肉色和/或解冻损失等几个肉质性状相关。这项研究揭示了统计学证据，除了这些基因的功能关系，WHC先前证明的表达分析。这项研究揭示了位置和遗传统计学证据的遗传变异的链接在这些位点或接近他们，并促进这六个候选基因作为功能和/或位置候选基因的肉质性状。
Six genes that were known to exhibit expression levels that are correlated to drip loss BVES, SLC3A2, ZDHHC5, CS, COQ9, and EGFR have been for candidate gene analysis. Based on in silico analysis SNPs were detected, confirmed by sequencing, and used for genotyping. The SNPs were genotyped in about 1,800 animals from six pig populations including commercial herds of Pietrain (PI) and German Landrace (DL), different commercial herds of Pietrain x (German Large White x German Landrace) (PIF1(a/b/c)), and one experimental F-2-population Duroc x Pietrain (DUPI). Comparative and genetic mapping established the location of BVES on SSC1, of SLC3A2 and ZDHHC5 on SSC2, of CS on SSC5, of COQ9 on SSC6 and of EGFR on SSC9, respectively, coinciding with QTL regions for carcass and meat quality traits. BVES, SLC3A2, and CS revealed association at least with drip loss and with several other measures of water holding capacity (WHC). Moreover, COQ9 and EGFR were associated with several meat quality traits such as meat color and/or thawing loss. This study reveals statistic evidence in addition to the functional relationship of these genes to WHC previously evidenced by expression analysis. This study reveals positional and genetic statistical evidence for a link of genetic variation at these loci or close to them and promotes those six candidate genes as functional and/or positional candidate genes for meat quality traits.