Membrane-based plasma collection device for point-of-care diagnosis of HIV
Membrane-based plasma collection device for point-of-care diagnosis of HIV
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DOI：
10.1016/j.jviromet.2011.01.003
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发表时间：
2011-04-01
影响因子：
3.1
通讯作者：
Kelso, David M.
中科院分区：
文献类型：
--
作者：
Nabatiyan, Arman;Parpia, Zaheer A.;Kelso, David M.
开发用于检测疾病分析物的即时检验的主要要求是需要以有效和快速的方式从全血中分离血浆。此外，分离的血浆必须能够有效地吸附目标分析物，并有效地用作物理基质，以提供用于下游诊断分析的纯血浆的等效物。此外，许多应用需要使用热休克来释放在收集的血浆中发现的免疫复合抗原。报道了一种用于从与热休克相容的全血样品中快速有效地收集血浆的基于膜的过滤方法。以儿科人类免疫缺陷病毒为例，该设备在采集后洗脱100%的输入p24核心抗原，并能够对血浆样本进行与纯血浆处理相同的热休克处理。(C)2011爱思唯尔有限公司版权所有。
A major requirement for the development of point-of-care tests for the detection of disease analytes is the need to separate plasma from whole blood in an efficient and rapid manner. Furthermore, the separated plasma must be able to elute efficiently the analyte of interest and serve effectively as a physical matrix to deliver the equivalent of neat plasma for downstream diagnostic analysis. Additionally, many applications require the use of heat shock to liberate immunocomplexed antigen found in the collected plasma. A membrane-based filter method is reported for rapid and efficient collection of plasma from a whole blood sample that is compatible with heat shock. Using pediatric human immunodeficiency virus as an example, this device elutes 100% of the input p24 core antigen post-collection and enables heat shock of plasma samples identical to neat plasma treatment. (C) 2011 Elsevier B.V. All rights reserved.