Cottontail rabbit papillomavirus-specific transcripts in transplantable tumors with integrated DNA.
Cottontail rabbit papillomavirus-specific transcripts in transplantable tumors with integrated DNA.
复制标题
具有整合 DNA 的可移植肿瘤中棉尾兔乳头瘤病毒特异性转录本。
DOI：
10.1016/0042-6822(84)90362-3
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发表时间：
1984
期刊：
影响因子：
3.7
通讯作者：
Wettstein,FO
中科院分区：
文献类型：
--
作者：
Nasseri,M;Wettstein,FO
分析了一株可移植性癌细胞系的病毒DNA和病毒转录物。两种肿瘤系都含有相同数量的基因拷贝/细胞的专门整合的病毒DNA。整合是在头-尾串联重复序列中，在可能的三个位点中，并且至少两个整合位点是常见的。在这两种肿瘤中，大约一半的病毒基因组都有90 bp的缺失。共同的整合位点和缺失表明这两个肿瘤系来源于相同的亲本肿瘤。肿瘤含有与家兔乳头状瘤和原发性癌中相同的1.3和2.0kb的主要病毒转录物[M. Nasseri，F. O. Wettstein和J. G. Stevens，J.Virol.44，263-268（1982）]。然而，癌细胞系在它们的次要转录物上不同。B17有四个5.2、3.8、3.6和3.0 kb的次要转录物，B12有两个4.8和3.0 kb的转录物。4.8-kb的转录物是相对主要的，并且似乎与棉尾兔的产病毒肿瘤中存在的主要转录物相同[M. Nasseri和F. O. Wettstein，J.Virol.51，706-712（1984）]。
The viral DNA and viral transcripts of a Vx2 and a Vx7 transplantable carcinoma lines were analyzed. Both tumor lines contain equivalent numbers of gene copies per cell of exclusively integrated viral DNA. Integration is in head-to-tail tandem repeats, in probably three sites and at least two integration sites are common. In both tumors, about half of the viral genomes share a 90-bp deletion. Common integration sites and deletions suggest that the two tumor lines were derived from the same parent tumor. The tumors contain the same major viral transcripts of 1.3 and 2.0 kb also found in both papillomas and primary carcinomas of domestic rabbits [M. Nasseri, F. O. Wettstein, and J. G. Stevens,J. Virol.44, 263–268 (1982)]. However, the carcinoma lines differ in their minor transcripts. Vx7 has four minor transcripts of 5.2, 3.8, 3.6, and 3.0 kb and Vx2 has two of 4.8 and 3.0 kb. The 4.8-kb transcript is relatively major and appears to be identical to a major transcript present in virus-producing tumors of cottontail rabbits [M. Nasseri and F. O. Wettstein,J. Virol.51, 706–712 (1984)].