Gap junctions from the lens: purification and characterization by chemical crosslinking reagent.
Gap junctions from the lens: purification and characterization by chemical crosslinking reagent.
复制标题
透镜间隙连接：通过化学交联试剂进行纯化和表征。
DOI：
10.1016/0006-291x(81)91748-4
复制
发表时间：
1981
影响因子：
3.1
通讯作者：
Hansen,JS
中科院分区：
文献类型：
--
作者：
Takemoto,LJ;Hansen,JS
通过透射电子显微镜对来自鸡晶状体的间隙连接制剂进行定量表明，95.0%的膜双层材料是三层结构的形式。该制剂由 26K 的主要多肽成分以及 49K、46K 和 22K-15K 的次要成分组成。用氧化剂处理导致产生涉及26K和46K组分的明显同源寡聚复合物。这些结果证明26K多肽是高度纯化的晶状体间隙连接制剂的主要成分。此外，他们证明这个 26K 成分加上一个额外的 46K 成分都参与完整连接复合体中广泛的最近邻相互作用。
Quantitation of gap junction preparations from chick lens by transmission electron microscopy has indicated that 95.0% of the membrane bilayer material is in the form trilayer structures. The preparations were comprised of a major polypeptide component of 26K, as well as minor components of 49K, 46K and 22K–15K. Treatment with oxidizing agent resulted in the production of apparent homo-oligomeric complexes involving the 26K and 46K components. These results demonstrate that the 26K polypeptide is the major component of highly purified preparations of lens gap junctions. Furthermore, they demonstrate that this 26K component plus an additional 46K component are both involved in extensive nearest-neighbor interactions in the intact junctional complex.