Myonectin inhibits the differentiation of osteoblasts and osteoclasts in mouse cells
Myonectin inhibits the differentiation of osteoblasts and osteoclasts in mouse cells
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DOI：
10.1016/j.heliyon.2020.e03967
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发表时间：
2020-05-01
期刊：
影响因子：
4
通讯作者：
Kaji, Hiroshi
中科院分区：
文献类型：
--
作者：
Kawaguchi, Miku;Kawao, Naoyuki;Kaji, Hiroshi
肌粘连蛋白是一种肌细胞因子，参与糖尿病和肥胖的病理生理过程，多种肌细胞因子参与骨骼肌和骨之间的相互作用。然而，肌粘连蛋白在骨中的作用仍然未知。因此，我们在体外研究了肌粘连蛋白对小鼠成骨细胞和破骨细胞分化的影响。肌粘连蛋白显著抑制小鼠成骨细胞成骨基因的mRNA水平和碱性磷酸酶（ALP）活性。对于破骨细胞，肌粘连蛋白显著抑制破骨细胞形成以及小鼠单核细胞RAW 264.7细胞中受体激活因子κ B配体（RANKL）增强的破骨细胞相关基因的mRNA水平。此外，在巨噬细胞集落刺激因子和RANKL存在下，肌连蛋白显著抑制小鼠骨髓细胞破骨细胞形成。另一方面，肌粘连蛋白显着抑制RAW264.7细胞中RANKL诱导的耗氧率和过氧化物酶体增殖物激活受体γ辅激活因子-1 β mRNA水平，尽管肌粘连蛋白不影响小鼠成骨细胞中的这些线粒体生物发生参数。结论：本研究表明，肌粘连蛋白抑制小鼠成骨细胞的分化和ALP活性。此外，肌粘连蛋白抑制破骨细胞分化从小鼠骨髓和RAW 264.7细胞部分通过抑制线粒体生物合成。
Myonectin is a myokine, which is involved in the pathophysiology of diabetes and obesity, and various myokines are involved in the interactions between skeletal muscle and bone. However, roles of myonectin in bone have still remained unknown. We therefore examined the effects of myonectin on mouse osteoblast and osteoclast differentiation in vitro. Myonectin significantly suppressed the mRNA levels of osteogenic genes and alkaline phosphatase (ALP) activity in mouse osteoblasts. As for osteoclasts, myonectin significantly suppressed osteoclast formation as well as the mRNA levels of osteoclast-related genes enhanced by receptor activator nuclear factor kappa B ligand (RANKL) from mouse monocytic RAW264.7 cells. Moreover, myonectin significantly suppressed osteoclast formation from mouse bone marrow cells in the presence of macrophage-colony stimulating factor and RANKL. On the other hand, myonectin significantly suppressed RANKL-induced oxygen consumption rate and peroxisome proliferator-activated receptor gamma coactivator-1 beta mRNA levels in RAW264.7 cells, although myonectin did not affect these mitochondrial biogenesis parameters in mouse osteoblasts. In conclusion, the present study demonstrated that myonectin suppresses the differentiation and ALP activity in mouse osteoblasts. Moreover, myonectin suppressed osteoclast differentiation from mouse bone marrow and RAW264.7 cells partly through an inhibition of mitochondrial biogenesis.