Detection of two heme oxygenase isoforms in the human testis.
Detection of two heme oxygenase isoforms in the human testis.
复制标题
检测人类睾丸中的两种血红素加氧酶亚型。
DOI：
10.1016/0006-291x(88)90682-1
复制
发表时间：
1988
影响因子：
3.1
通讯作者：
Maines,MD
中科院分区：
文献类型：
--
作者：
Trakshel,GM;Maines,MD
这项研究表明，血红素加氧酶的多样性是共同的大鼠和人体组织。然而，人和大鼠的同工酶是具有某些共同特征的异质蛋白质。在人类睾丸中发现了两种形式的血红素加氧酶，HO-1和HO-2。HO-2型为优势型。人和大鼠HO-1的色谱行为和分子量不同;人HO-1是一个较大的分子（35，400 vs 30，000）。然而，这两种形式的相似之处在于，免疫化学上人HO-1对大鼠HO-1抗体表现出反应性。人和大鼠HO-2的色谱行为也不同，仅显示出弱的免疫交叉反应性。人类和大鼠HO-1的大小基本相同。与大鼠器官一样，人睾丸微粒体细胞色素P-450含量与血红素加氧酶活性成反比。
This study shows heme oxygenase multiplicity is common to rat and human tissues. The isozymes in man and rat, however, are heterogenous proteins that share certain characteristics. Two forms of heme oxygenase, HO-1 and HO-2, were identified in human testis. HO-2 form was the prevalent form. Human and rat HO-1 differed in chromatographic behavior and molecular weight; human HO-1 was a larger molecule (35,400 vs 30,000). The two forms, however, were similar in that immunochemically human HO-1 exhibited reactivity toward antibody to rat HO-1. Human and rat HO-2 also were dissimilar in chromatographic behavior and showed only a weak immunological cross-reactivity. Human and rat HO-1 were essentially the same size. As in rat organs, the microsomal cytochrome P-450 content in human testis was reciprocal to heme oxygenase activity.