Linker-free covalent immobilization of heparin, SDF-1α, and CD47 on PTFE surface for antithrombogenicity, endothelialization and anti-inflammation
Linker-free covalent immobilization of heparin, SDF-1α, and CD47 on PTFE surface for antithrombogenicity, endothelialization and anti-inflammation
复制标题
将肝素、SDF-1 α 和 CD47 无接头共价固定在 PTFE 表面上，以实现抗血栓形成、内皮化和抗炎
DOI：
10.1016/j.biomaterials.2017.06.023
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发表时间：
2017-09-01
期刊：
影响因子：
14
通讯作者：
Chu, Paul K.
中科院分区：
文献类型：
--
作者：
Gao, Ang;Hang, Ruiqiang;Chu, Paul K.
生物医用聚四氟乙烯（PTFE）制成的小直径血管移植物因血栓形成和内膜增生而长期通畅率较低，这可能与材料的内皮化不足和慢性炎症有关。因此，非常需要 PTFE 接枝物的生物功能化来克服这些缺点。在这项研究中，描述了一种多功能的“植入-孵化”方法，其中生物医学 PTFE 最初通过等离子体浸没离子注入 (PHI) 进行改性。经过N-2 PIII处理后，生物医用PTFE的表面变得粗糙并具有纳米结构，更重要的是，表面下方产生的丰富自由基不断迁移到表面并与环境分子发生反应。利用这种机制，通过简单的溶液浸泡，可以将具有不同功能的各种生物分子稳定地固定在PITE表面。例如，三种典型的生物分子：肝素、SDF-1 α 和 CD47，共价接枝到 PTFE 上。除了保留生物活性外，表面功能化的PTFE还表现出降低的血栓形成性，促进内皮祖细胞的募集，甚至减轻单核巨噬细胞的炎症免疫反应，因此有望开发具有良好长期通畅性的小直径人工血管移植物。 (C) 2017 Elsevier Ltd. 保留所有权利。
Small-diameter vascular grafts made of biomedical polytetrafluoroethylene (PTFE) suffer from the poor long-term patency rate originating from thrombosis and intimal hyperplasia, which can be ascribed to the insufficient endothelialization and chronic inflammation of the materials. Hence, biofunctionalization of PTFE grafts is highly desirable to circumvent these disadvantages. In this study, a versatile "implantation-incubation" approach in which the biomedical PTFE is initially modified by plasma immersion ion implantation (PHI) is described. After the N-2 PIII treatment, the surface of biomedical PTFE is roughened with nanostructures and more importantly, the abundant free radicals generated underneath the surface continuously migrate to the surface and react with environmental molecules. Taking advantage of this mechanism, various biomolecules with different functions can be steadily immobilized on the surface of PITE by simple solution immersion. As examples, three typical biomolecules, heparin, SDF-1 alpha, and CD47, are covalently grafted onto the PTFE. In addition to retaining the bioactivity, the surface-functionalized PTFE exhibits reduced thrombogenicity, facilitates the recruitment of endothelial progenitor cells, and even alleviates the inflammatory immune responses of monocytes-macrophages and is thus promising to the development of small-diameter prosthetic vascular grafts with good long-term patency. (C) 2017 Elsevier Ltd. All rights reserved.