Adamts5, the gene encoding a proteoglycan-degrading metalloprotease, is expressed by specific cell lineages during mouse embryonic development and in adult tissues.
Adamts5, the gene encoding a proteoglycan-degrading metalloprotease, is expressed by specific cell lineages during mouse embryonic development and in adult tissues.
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DOI：
10.1016/j.gep.2009.02.006
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发表时间：
2009-06
期刊：
影响因子：
--
通讯作者：
Apte SS
中科院分区：
文献类型：
--
作者：
McCulloch DR;Le Goff C;Bhatt S;Dixon LJ;Sandy JD;Apte SS
分泌的金属蛋白酶ADAMTS5与关节炎中软骨蛋白多糖聚集蛋白的破坏有关，但其生理功能尚不清楚。因此，其在胚胎发生和成人组织中的表达谱引起了相当大的兴趣。通过插入Adamts5位点的LacZ盒激活β-半乳糖苷酶（β-gal）组织化学，并通过Adamts5 cRNA探针和Adamts5免疫组织化学原位杂交验证，用于分析小鼠胚胎发生和成年小鼠组织中Adamts5的表达。胚胎表达在妊娠11.5天（E11.5）之前很少，仅在妊娠9.5天发育中的脑底板中发现。e11.5后在脑组织中继续表达，尤其是在脉络膜丛、周围神经、背根神经节、脑神经神经节、脊神经和脑神经以及肠道神经丛中。除神经外，发育中的肢体在骨骼肌（来自E13.5）、肌腱（来自E16.5）和发育中的自足指间质（E13.5 - 15.5）中也有Adamts5表达。在成人组织中，Adamts5在动脉平滑肌细胞、腹膜、心包和胸膜腔内壁间皮、支气管和胰管平滑肌细胞、肾脏肾球系膜细胞、背根神经节以及周围和自主神经系统的雪旺细胞中均有组成性表达。Adamts5在神经肌肉发育和平滑肌细胞中的表达与广泛分布的蛋白聚糖versican一致，后者是Adamts5的底物。这些观察结果表明，可以在发育和生理作用中寻找这种蛋白酶的主要背景。
The secreted metalloprotease ADAMTS5 is implicated in destruction of the cartilage proteoglycan aggrecan in arthritis, but its physiological functions are unknown. Its expression profile during embryogenesis and in adult tissues is therefore of considerable interest. β-galactosidase (β-gal) histochemistry, enabled by a LacZ cassette inserted in the Adamts5 locus, and validated by in situ hybridization with an Adamts5 cRNA probe and ADAMTS5 immunohistochemistry, was used to profile Adamts5 expression during mouse embryogenesis and in adult mouse tissues. Embryonic expression was scarce prior to 11.5 days of gestation (E11.5) and noted only in the floor plate of the developing brain at E9.5. After E 11.5 there was continued expression in brain, especially in the choroid plexus, peripheral nerves, dorsal root ganglia, cranial nerve ganglia, spinal and cranial nerves, and neural plexuses of the gut. In addition to nerves, developing limbs have Adamts5 expression in skeletal muscle (from E13.5), tendons (from E16.5), and inter-digital mesenchyme of the developing autopod (E13.5–15.5). In adult tissues, there is constitutive Adamts5 expression in arterial smooth muscle cells, mesothelium lining the peritoneal, pericardial and pleural cavities, smooth muscle cells in bronchii and pancreatic ducts, glomerular mesangial cells in the kidney, dorsal root ganglia, and in Schwann cells of the peripheral and autonomic nervous system. Expression of Adamts5 during neuromuscular development and in smooth muscle cells coincides with the broadly distributed proteoglycan versican, an ADAMTS5 substrate. These observations suggest the major contexts in which developmental and physiological roles could be sought for this protease.
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影响因子：
4.5
作者：
Silver DL;Hou L;Somerville R;Young ME;Apte SS;Pavan WJ
通讯作者：
Pavan WJ
影响因子：
16.2
作者：
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通讯作者：
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影响因子：
4.8
作者：
Dutt, S;Kléber, M;Zimmermann, DR
通讯作者：
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影响因子：
4.8
作者：
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通讯作者：
Burn, TC
影响因子：
2.5
作者：
Kern, Christine B.;Norris, Russell A.;Mjaatvedt, Corey H.
通讯作者：
Mjaatvedt, Corey H.