Immunogold localization of callose and other plant cell wall components in soybean roots infected with the oomycete Phytophthora sojae
Immunogold localization of callose and other plant cell wall components in soybean roots infected with the oomycete Phytophthora sojae
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感染大豆疫霉的大豆根中胼胝质和其他植物细胞壁成分的免疫金定位
DOI：
10.1139/b97-865
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
M. Hahn
中科院分区：
文献类型：
--
作者：
K. Enkerli;C. Mims;M. Hahn
用免疫标记和透射电子显微镜技术研究了接种大豆疫霉2号生理小种的感病和抗病大豆植株根部附壁的化学性质，并对与大豆疫霉吸器有关的吸器外基质进行了检测。使用了抗(1-3)-P-葡聚糖和存在于木葡聚糖和鼠李半乳糖醛酸链I中的末端a-岩藻糖基表位的抗体，以及存在于阿拉伯半乳糖蛋白中的阿拉伯糖基化的(1-6)-0-半乳聚糖表位的抗体。(1+3)-0-葡聚糖、木葡聚糖和阿拉伯半乳糖蛋白均定位于所有的壁对位，无论这些对位在接种多长时间后形成，也不管植物对黄豆赤霉病是敏感还是抗病。(1-3)-0-葡聚糖也存在于真菌壁和寄主细胞胞间连丝中。四种抗体均未标记吸器外基质。抗阿拉伯半乳糖蛋白的抗体识别寄主质膜，但不识别与吸器外基质相关的内陷的寄主质膜。这一结果表明，一旦宿主质膜成为吸器外膜，其性质或组成可能会发生局部变化。
Immunolabeling and transmission electron microscopic techniques were used to investigate the chemical nature of wall appositions in roots of susceptible and resistant soybean plants inoculated with Phytophthorn sojae race 2. The extrahaustorial matrix associated with the haustorium of Plzyrophthora sojae also was examined. Antibodies against (1-3)-P-glucan, a terminal a-fucosyl-containing epitope present in xyloglucan and rhamnogalacturonan I, and an arabinosylated (1-6)-0-galactan epitope present in arabinogalactan proteins were used. (1+3)-0-Glucan (callose), xyloglucan, and arabinogalactan proteins were found to be localized in all wall appositions regardless of how long after inoculation the appositions developed or whether plants were susceptible or resistant to Phyrophrhorn sojae. (1-3)-0-Glucan also was found in fungal walls and at host cell plasmodesmata. None of the four antibodies labeled the extrahaustorial matrix. The antibody against arabinogalactan protein recognized the host plasma membrane, but not the invaginated host plasma membrane associated with the extrahaustorial matrix. This result indicates that the properties or the composition of the host plasma membrane may change locally once it becomes an extrahaustorial membrane.