HMGIC expression in human adult and fetal tissues and in uterine leiomyomata
HMGIC expression in human adult and fetal tissues and in uterine leiomyomata
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DOI：
10.1002/(sici)1098-2264(199908)25:4
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发表时间：
1999-08-01
影响因子：
3.7
通讯作者：
Morton, CC
中科院分区：
文献类型：
--
作者：
Gattas, GJF;Quade, BJ;Morton, CC
高迁移率群 (HMG) 蛋白基因 HMGIC 定位于 12 号染色体 q15 条带，该区域经常在良性间质肿瘤（包括子宫平滑肌瘤）中重排。尽管一些证据表明 HMGIC 在细胞增殖调节中发挥作用，但 HMGIC 在这些肿瘤发生或进展中的确切功能仍不清楚。我们通过 Northern blot 和逆转录聚合酶链反应研究了 17 种胎儿组织（肾上腺、主动脉、骨、脑、心脏、肠、肾、肝、肺、肌肉、卵巢、胎盘、皮肤、脾、胃、睾丸和子宫）和 10 种成人组织（主动脉、脑、小脑、脂肪、肾、肝、肺、淋巴结、子宫肌层和脊髓）中的 HMGIC 表达。 (RT-PCR) 检测。还对来自 11 个子宫肌瘤和 7 个正常匹配的子宫肌层或体外细胞培养物（绒毛膜绒毛、胎盘、子宫肌层、平滑肌瘤和皮肤）的肿瘤样本中的 HMGIC 基因表达进行了比较。该基因在所有测试的胎儿组织中表达，但仅在成人肺和肾中表达。 HMGIC 还在含有 t(12;14) 的平滑肌瘤肿瘤样品和所有体外细胞培养物中表达。 HMGIC 表达模式表明该基因在快速增殖的人类胎儿组织中很重要。平滑肌瘤中表达的恢复需要 HMGIC 的失调。体外细胞培养后也可以检测到 HMGIC 的转录本，这表明 HMGIC 的表达可能受到培养基和血清中存在的因素的影响。基因染色体癌症 25:316-322, 1999。(C) 1999 Wiley-Liss, Inc.
The high-mobility-group (HMG) protein gene, HMGIC, is localized to chromosome 12, band q15, a region often rearranged in benign mesenchymal tumors, including uterine leiomyomata. Although some evidence suggests a role in regulation of cell proliferation, the precise function of HMGIC in the development or progression of these tumors remains unclear. We investigated HMGIC expression in 17 fetal tissues (adrenal, aorta, bone, brain, heart, intestine, kidney, liver, lung, muscle, ovary, placenta, skin, spleen, stomach, testis, and uterus) and 10 adult tissues (aorta, brain, cerebellum, fat, kidney, liver, lung, lymph node, myometrium, and spinal cord) by Northern blot and reverse transcriptase-polymerase chain reaction (RT-PCR) assays. Comparisons between HMGIC gene expression in tumor samples from 11 uterine leiomyomata and 7 normal matched myometrium or in vitro cell cultures (chorionic villi, placenta, myometrium, leiomyoma, and skin) were also performed. The gene was expressed in all fetal tissues tested but only in adult lung and kidney. HMGIC was also expressed in leiomyoma tumor samples containing t(12; 14) and in all in vitro cell cultures. The pattern of HMGIC expression suggests that this gene is important in rapidly proliferating human fetal tissues. Restoration of expression in leiomyomata required dysregulation of HMGIC. Transcripts of HMGIC can also be detected after in vitro cell culture, suggesting that HMGIC expression may be affected by factors present in culture media and serum. Genes Chromosomes Cancer 25:316-322, 1999. (C) 1999 Wiley-Liss, Inc.