Vector potential of cockroaches for Helicobacter pylori infection
Vector potential of cockroaches for Helicobacter pylori infection
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DOI：
10.1111/j.1572-0241.2003.07516.x
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发表时间：
2003-07
影响因子：
9.8
通讯作者：
Shigeyoshi Imamura;M. Kita;Y. Yamaoka;Toshiro Yamamoto;A. Ishimaru;H. Konishi;Naoki Wakabayashi;S. Mitsufuji;T. Okanoue;J. Imanishi
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文献类型：
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作者：
Shigeyoshi Imamura;M. Kita;Y. Yamaoka;Toshiro Yamamoto;A. Ishimaru;H. Konishi;Naoki Wakabayashi;S. Mitsufuji;T. Okanoue;J. Imanishi
提示：人类感染幽门螺杆菌的途径仍不清楚。在本研究中，我们研究了蟑螂作为传播H。方法：我们使用一种常见的蟑螂（黑胸大蠊）。禁食3天后，将蟑螂置于含有新鲜生长的H. pylori（Sydney株）（攻击组）或无H. pylori感染（对照组）。攻击24小时后，将蟑螂移至消毒容器中，并提供无菌食物和水。采集蟑螂体表（腿、体）及排泄物进行细菌培养、快速尿素酶试验和聚合酶链反应（PCR）。幽门螺杆菌可从攻毒组的排泄物中培养24 h。快速尿素酶试验结果阳性，直至第3天，PCR分析为H。pylori DNA，直至第7天。与此相反，H.从蟑螂的外表面不能培养出幽门螺杆菌。快速尿素酶试验仅8 h阳性，PCR分析H.结论：蟑螂通常生活在不卫生的环境中，并可能污染食品和食品密封区，如茶水间。传播H.幽门螺杆菌感染可能是由于无意中摄入了被含有活幽门螺杆菌的蟑螂排泄物污染的食物。幽门。
OBJECTIVES:The routes of human infection with Helicobacter pylori remain unclear. In the present study, we examined cockroaches as possible vectors for transmission of H. pylori.METHODS:We used a common species of cockroach (Periplaneta fuliginosa). After a 3-day fast, cockroaches were placed on agar plates containing freshly grown H. pylori (Sydney strain) (challenge group) or on sterile agar plates without H. pylori (control group). After 24 h of challenge, cockroaches were moved to disinfected containers, and sterile food and water were provided. The external surfaces (legs and body) and excreta of the cockroaches were sampled for culture, rapid urease test, and polymerase chain reaction (PCR).RESULTS:H. pylori were culturable from the excreta of the challenge group for 24 h postchallenge. Positive rapid urease test results were obtained up to day 3, and PCR analysis was positive for H. pylori DNA up to day 7 from the excreta. In contrast, H. pylori were not culturable from the external surfaces of the cockroaches. The rapid urease test was positive for only 8 h, and PCR analysis was positive for H. pylori DNA for 1 day from the external surface.CONCLUSIONS:Cockroaches usually live in unsanitary environments and may contaminate foods and food containment areas such as pantries. Transmission of H. pylori infection could be achieved via inadvertent ingestion of foods contaminated with cockroach excreta containing viable H. pylori.