CRYO-ELECTRON MICROSCOPY OF VIRUSES
CRYO-ELECTRON MICROSCOPY OF VIRUSES
复制标题
病毒的冷冻电镜技术
DOI：
10.1038/308032a0
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发表时间：
1984-01-01
期刊：
影响因子：
64.8
通讯作者：
MCDOWALL, AW
中科院分区：
文献类型：
--
作者：
ADRIAN, M;DUBOCHET, J;MCDOWALL, AW
可以制备未固定、未染色和无支撑的病毒悬浮液的薄玻璃化层，以便在易于控制的条件下通过冷冻电镜进行观察。病毒颗粒似乎没有因脱水、冷冻或吸附到载体上而造成的损坏，而这些损坏是在制备用于常规电镜的生物样品时遇到的。玻璃化标本的冷冻电镜提供了高分辨率观察的可能性，与任何其他电镜方法相比都具有优势。 [本研究测试了动物病毒和噬菌体。]。
Thin vitrified layers of unfixed, unstained and unsupported virus suspensions can be prepared for observation by cryo-EM in easily controlled conditions. The viral particles appear free from the kind of damage caused by dehydration, freezing or adsorption to a support that is encountered in preparing biological samples for conventional EM. Cryo-EM of vitrified specimens offers possibilities for high resolution observations that compare favorably with any other EM method. [Animal viruses and bacteriophages were tested in this study.].