Pitx2 distinguishes subtypes of terminally differentiated neurons in the developing mouse neuroepithelium
Pitx2 distinguishes subtypes of terminally differentiated neurons in the developing mouse neuroepithelium
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DOI：
10.1006/dbio.2002.0835
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发表时间：
2002-12-01
影响因子：
2.7
通讯作者：
Camper, SA
中科院分区：
文献类型：
--
作者：
Martin, DM;Skidmore, JM;Camper, SA
Pitx2是一种同源结构域转录因子，对垂体、眼睛、心脏和牙齿的正常发育至关重要。在发育中的小鼠大脑中，Pitx2 (Rieg, Ptx2, Otlx2, Brx1) mRNA在间脑，中脑和斜脑膜的离散区域表达。虽然先前的报道提供了Pitx2 mRNA在大脑中表达的时间和区域特异性的概述，但表达Pitx2的确切细胞类型尚不清楚。在本研究中，我们利用多种细胞增殖和分化标记分析了发育中的e10.5 -e14.5小鼠中枢神经系统单个细胞中Pitx2 mRNA和Pitx2蛋白的表达。我们在巢蛋白阳性的神经祖细胞和有丝分裂后发育中的神经元中发现了Pitx2的表达。在间脑中，PITX2表达于丘脑内限定带和乳头区神经元，以及不规则带γ -氨基丁酸（GABA）产生神经元。在中脑，pitx2标记的核也出现在分化的神经元中，其中一些是gaba能的，注定占据上丘。我们的研究结果表明，PITX2在有丝分裂后神经元中的表达可能有助于gaba能和其他分化神经元表型的发展。(C) 2002 Elsevier Science （USA）。
Pitx2, a homeodomain transcription factor, is essential for normal development of pituitary, eyes, heart, and teeth. In the developing mouse brain, Pitx2 (Rieg, Ptx2, Otlx2, Brx1) mRNA is expressed in discrete regions of the diencephalon, mesencephalon, and rhombencephalon. While prior reports have provided an overview of the temporal and regional specificity of Pitx2 mRNA expression in the brain, the precise cell types that express PITX2 are not known. In this study, we analyzed Pitx2 mRNA and PITX2 protein expression in individual cells of the developing e 10.5-e14.5 mouse CNS using multiple markers of cellular proliferation and differentiation. We identified Pitx2 expression in nestin-positive neural progenitors and in postmitotic, developing neurons. In the diencephalon, PITX2 is expressed in neurons of the zona limitans intrathalamica and mammillary region and in gamma-aminobutyric acid (GABA)-producing neurons of the zona incerta. in the mesencephalon, PITX2-labeled nuclei also appear in differentiated neurons, some of which are GABAergic and destined to occupy superior colliculus. Our results suggest that PITX2 expression in postmitotic neurons may contribute to development of GABAergic and other differentiated neuronal phenotypes. (C) 2002 Elsevier Science (USA).