Comparison of rapid centrifugation assay with conventional tissue culture method for isolation of dengue 2 virus in C6/36-HT cells
Comparison of rapid centrifugation assay with conventional tissue culture method for isolation of dengue 2 virus in C6/36-HT cells
复制标题
DOI：
10.1128/jcm.38.9.3508-3510.2000
复制
发表时间：
2000-09-01
影响因子：
9.4
通讯作者：
Kourí, G
中科院分区：
文献类型：
--
作者：
Roche, RR;Alvarez, M;Kourí, G
对登革热和登革出血热/登革休克综合征致死病例的血清和尸检样本中的登革病毒分离，将快速离心法与常规试管细胞培养法进行了比较。与传统方法相比，快速离心法可以从16.6%的样本中分离出病毒，并缩短了登革病毒检测的时间。最后，它允许在5例死亡病例的42.8%的组织样本中分离出登革热2型病毒。我们的研究结果表明，快速离心法可能是有用的检测登革病毒在临床标本。
A rapid centrifugation assay was compared with conventional tube cell culture for dengue virus isolation in both sera and autopsy samples from dengue and dengue hemorrhagic fever/dengue shock syndrome fatal cases. The rapid centrifugation assay allowed isolation of virus from 16.6% more samples than the conventional method, and it shortened the time for dengue virus detection. Finally, it allowed the isolation of dengue 2 virus in 42.8% of tissue samples from five fatal cases. Our results suggest that the rapid centrifugation assay may be useful for detection of dengue virus in clinical specimens.