Calcification of senile cataractous lens determined by Fourier transform infrared (FTIR) and Raman microspectroscopies
Calcification of senile cataractous lens determined by Fourier transform infrared (FTIR) and Raman microspectroscopies
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DOI：
10.1111/j.1365-2818.2005.01491.x
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发表时间：
2005-07-01
影响因子：
2
通讯作者：
Lin, SY
中科院分区：
文献类型：
--
作者：
Chen, KH;Cheng, WT;Lin, SY
从一位79岁男性患者的老年性白内障透镜（CL）表面上的钙化斑块被鉴定，并使用傅立叶变换红外（FTIR）和共焦拉曼显微光谱对其化学成分进行定量。选择同一CL和羟基磷灰石（HA）的非钙化区作为对照。在钙化斑块和HA的红外（IR）光谱中清楚地显示了几个独特的吸收带，在960、1034和1090 cm（-1）处归属于磷酸盐的nu（1）和nu（3）伸缩模式，在875 cm（-1）处归属于碳酸盐。在钙化斑块和HA的拉曼光谱中也证实了由于磷酸盐的nu（1）伸缩模式而在961 cm（-1）处的峰。在透镜蛋白内形成的钙化斑主要由成熟的HA组成，其中A型碳酸磷灰石（11.4%）、B型碳酸磷灰石（55.6%）和易溶的表面碳酸根离子（33.0%）存在。易溶碳酸盐的含量较高，意味着该钙化透镜中的钙化或矿化不完全且仍在进行中。此外，钙化似乎不影响透镜蛋白的二级结构，因为在非钙化区和钙化斑块中的透镜蛋白的IR和拉曼光谱是相似的。结果表明，显微FTIR和拉曼光谱都易于执行，并能够确定钙化CL的化学组成。
A calcified plaque on the surface of a senile cataractous lens (CL) isolated from a 79-year-old male patient was identified and its chemical composition quantified using Fourier transform infrared (FTIR) and confocal Raman microspectroscopies. The noncalcified area of the same CL and hydroxyapatite (HA) were selected as a control. Several unique absorption bands, at 960, 1034 and 1090 cm(-1) assigned to the nu(1) and nu(3) stretching modes of phosphate and at 875 cm(-1) attributed to carbonate, were clearly displayed in the infrared (IR) spectra of calcified plaque and HA. A peak at 961 cm(-1) due to the nu(1) stretching mode of phosphate was also evidenced in the Raman spectra of calcified plaque and HA. The calcified plaque formed within the lens protein was found to mainly consist of a mature HA, in which type-A carbonate apatites (11.4%), type-B carbonate apatites (55.6%) and liable surface carbonate ions (33.0%) were presented. A higher content of the liable carbonate implies that the calcification or mineralization in this calcified lens was incomplete and still in progress. Moreover, calcification seems not to influence the secondary structure of lens protein because both IR and Raman spectra for the lens protein in the noncalcified area and calcified plaque were similar. The result suggests that both microscopic FTIR and Raman spectroscopies were easy to perform and capable of determination of the chemical composition of a calcified CL.