Identification of genes responsive to low intensity pulsed ultrasound in a human leukemia cell line Molt-4.
Identification of genes responsive to low intensity pulsed ultrasound in a human leukemia cell line Molt-4.
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DOI：
10.1016/j.canlet.2006.02.011
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发表时间：
2007-02
期刊：
影响因子：
9.7
通讯作者：
Y. Tabuchi;H. Ando;I. Takasaki;L. B. Feril;Qing‐Li Zhao;R. Ogawa;N. Kudo;K. Tachibana;T. Kondo
中科院分区：
文献类型：
--
作者：
Y. Tabuchi;H. Ando;I. Takasaki;L. B. Feril;Qing‐Li Zhao;R. Ogawa;N. Kudo;K. Tachibana;T. Kondo
我们研究了非热低强度脉冲超声处理的人白血病Molt-4细胞的基因表达。在0.3W/cm 2脉冲超声处理后6小时，观察到细胞凋亡（24± 3.3%，平均值±SD）和最小的细胞溶解。在分析的大约16，600个基因中，BCL 2相关的产氧基因3（BAG 3）、DnaJ（Hsp 40）同源物、亚家族B、成员1（DNAJB 1）、热休克70 kDa蛋白1 B（HSPA 1 B）、和热休克蛋白6（HSPA 6）的表达水平增加，而异戊烯基二磷酸δ异构酶（IDI 1）和3-羟基-3-甲基戊二酰辅酶A合酶1（HMGCS 1）的表达水平增加。显示在超声处理后3小时细胞中的水平降低。通过实时定量聚合酶链反应证实了这六个基因的表达水平。据我们所知，这是第一个报告的DNA微阵列分析的基因，差异表达的非热低强度脉冲超声诱导的人白血病细胞凋亡的反应。本研究结果将为进一步了解低强度脉冲超声作用的分子机制以及机械剪切应力作用的分子机制奠定基础。
We examined the gene expression of human leukemia Molt-4 cells treated with non-thermal low intensity pulsed ultrasound. Six hours after 0.3W/cm2pulsed ultrasound treatment, apoptosis (24±3.3%, mean±SD) with minimal cell lysis was observed. Of approximately 16,600 genes analyzed, BCL2-associated athanogene 3 (BAG3), DnaJ (Hsp40) homolog, subfamily B, member 1 (DNAJB1), heat shock 70kDa protein 1B (HSPA1B), and heat shock 70kDa protein 6 (HSPA6) showed increased levels of expression while isopentenyl-diphosphate delta isomerase (IDI1) and 3-hydroxy-3-methylglutaryl–coenzyme A synthase 1 (HMGCS1) showed decreased levels in the cells 3h after the ultrasound treatment. The expression levels of these six genes were confirmed by a real-time quantitative polymerase chain reaction. To our knowledge, this is the first report of DNA microarray analysis of genes that are differentially expressed in response to apoptosis induced by non-thermal low intensity pulsed ultrasound in human leukemia cells. The present results will provide a basis for further understanding of the molecular mechanisms of effects of not only low intensity pulsed ultrasound but also that of mechanical shear stress in the cells.