Chemical carcinogen-mouse mammary tumor virus interactions in cell transformation.
Chemical carcinogen-mouse mammary tumor virus interactions in cell transformation.
复制标题
化学致癌物-小鼠乳腺肿瘤病毒在细胞转化中的相互作用。
DOI：
10.1007/bf02617995
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Fisher,PB
中科院分区：
文献类型：
--
作者：
Howard,DK;Schlom,J;Fisher,PB
我们已经研究了乳腺细胞转化的过程中，在体外使用一个假定的上皮细胞系，C57 MG，来自正常乳腺的单细胞克隆（克隆18）;小鼠乳腺肿瘤病毒（MMTV）的宿主范围变异体（RIII）vp 4;和有效的启动致癌物7，12-二甲基苯并（a）蒽（DMBA）。经过几个系列的传代培养，细胞与病毒，然后与致癌剂处理表现出改变（转化）的形态，锚定独立性的显着增加，暴露于细胞松弛素B后，在多核化的增加，在低钙（0.01 mM）培养基中增殖的能力增强，和致瘤性皮下接种到无胸腺（裸）小鼠。尽管在单独用MMTV或DMBA处理的培养物中以及在感染MMTV之前暴露于DMBA的培养物中也观察到了其中一些表型改变，但仅在首先感染MMTV然后暴露于DMBA的克隆细胞的大量培养物中观察到了增强的细胞松弛素B多核化和致瘤性。这第一次证明，假定的乳腺上皮细胞暴露于MMTV，然后是DMBA，但不是单独的试剂或DMBA，然后是MMTV，导致这些细胞的恶性转化。
We have studied the process of mammary cell transformation in vitro using a single cell clone (Clone 18) from a presumptive epithelial cell line, C57MG, derived from a normal mammary gland; a mouse mammary tumor virus (MMTV) host-range variant (RIII)vp4; and the potent initiating carcinogen 7,12-dimethylbenz(a)anthracene (DMBA). After several serial subcultures, cells treated with virus and then with carcinogen exhibited an altered (transformed) morphology, a dramatic increase in anchorage independence, an increase in multinucleation after exposure to cytochalasin B, an enhanced ability to proliferate in low Ca2+(0.01 mM) medium, and tumorigenicity when inoculated subcutaneously into athymic (nude) mice. Although some of these phenotypic alterations were observed also in cultures treated singly with MMTV or DMBA and in cultures exposed to DMBA before infection with MMTV, enhanced cytochalasin B multinucleation and tumorigenicity were properties observed only in mass cultures of cloned cells first infected with MMTV and then exposed to DMBA. This demonstrates for the first time that exposure of presumptive mammary epithelial cells to MMTV followed by DMBA, but not to either agent alone or to DMBA followed by MMTV, results in malignant transformation of these cells.