A synergistic bone sparing effect of curcumin and alendronate in ovariectomized rat
A synergistic bone sparing effect of curcumin and alendronate in ovariectomized rat
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DOI：
10.1007/s00701-012-1516-9
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发表时间：
2012-12-01
影响因子：
2.4
通讯作者：
Sung, Joo-Kyung
中科院分区：
文献类型：
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作者：
Cho, Dae-Chul;Kim, Kyoung-Tae;Sung, Joo-Kyung
本研究旨在评价姜黄素和阿仑磷酸钠联合治疗对去卵巢后大鼠骨重建的影响。将去卵巢动物随机分为4组：未治疗组、姜黄素组、阿仑膦酸组和联合治疗组。术后8周和12周，每组大鼠均行安乐死。检测血清骨钙素、碱性磷酸酶(ALP)及I型胶原C末端(CTX)端肽片段。用显微计算机断层扫描(CT)测量第4腰椎的骨形态计量学参数。实验组(姜黄素组、阿伦磷酸组、联合治疗组)骨转换生化指标明显低于未治疗组(p<；0.05)。联合治疗组12周时ALP和CTX-1浓度低于姜黄素组和阿仑膦酸钠组(p<；0.05)。联合治疗组8周时的骨密度和12周时的Cr.BMD均显著高于单纯姜黄素组(p=0.005和p=0.013)。三点弯曲试验显示，联合治疗组第4腰椎的最大负荷值明显大于单纯姜黄素组和单纯阿伦磷酸酯组(P<；0.05)。本研究表明，大剂量姜黄素和标准剂量阿伦磷酸钠联合治疗在抑制骨重建和提高骨机械强度方面优于姜黄素或阿伦磷酸钠单一治疗。
The purpose of this study was to evaluate the therapeutic effects of combination therapy with curcumin and alendronate on bone remodeling after ovariectomy in rats.Eighty female Sprague-Dawley rats underwent either a sham operation (the sham group) or bilateral ovariectomy (OVX). The ovariectomized animals were randomly distributed amongst four groups: untreated OVX group, curcumin-administered group, alendronate-administered group, and the combination therapy group. At 8 and 12 weeks after surgery, rats from each of the groups were euthanized. Serum biochemical markers of bone turnover, including osteocalcin and alkaline phosphatase (ALP), and the telopeptide fragment of type I collagen C-terminus (CTX) were analyzed. Bone histomorphometric parameters of the 4th lumbar vertebrae were determined by micro-computed tomography (CT). In addition, mechanical strength was determined by a three-point bending test.Serum biochemical markers of bone turnover in the experiment groups (curcumin administered group, alendronate administered group, and the combination therapy group) were significantly lower than in the untreated OVX group (p < 0.05). The combination therapy group had lower ALP and CTX-1 concentrations at 12 weeks, which were statistically significant compared with the curcumin only and the alendronate only group (p < 0.05). The combination therapy group had a significant increase in BMD at 8 weeks and Cr.BMD at 12 weeks compared with the curcumin-only group (p = 0.005 and p = 0.013, respectively). The three point bending test showed that the 4th lumbar vertebrae of the combination therapy group had a significantly greater maximal load value compared to that of the curcumin only and the alendronate only group (p < 0.05).The present study demonstrated that combination therapy with a high dose of curcumin and a standard dose of alendronate has therapeutic advantages over curcumin or alendronate monotherapy, in terms of the synergistic antiresorptive effect on bone remodeling, and improving bone mechanical strength.