THE INORGANIC PHOSPHATE AND PHOSPHOCREATINE OF BRAIN ESPECIALLY DURING METABOLISM INVITRO
THE INORGANIC PHOSPHATE AND PHOSPHOCREATINE OF BRAIN ESPECIALLY DURING METABOLISM INVITRO
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DOI：
10.1042/bj0480012
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发表时间：
1951-01-01
影响因子：
4.1
通讯作者：
CHESHIRE, JD
中科院分区：
文献类型：
--
作者：
MCILWAIN, H;BUCHEL, L;CHESHIRE, JD
用氢氧化钙-乙醇分离法测定豚鼠脑中无机磷和磷酸肌酸的含量。对磷酸盐和肌酸进行了估算。不经化学分离即可测定无机磷和磷酸肌酸不稳定的磷酸盐。豚鼠死后，与其他物种一样，大脑中的磷酸肌酸迅速下降，而无机磷迅速上升。在正常新陈代谢条件下切开大脑皮层，让脑片与葡萄糖一起呼吸时，无机磷会下降，而磷酸肌酸会上升，直到浓度升高。接近活体组织的正常组织。康涅狄格州。达到的程度随代谢混合物的不同而不同。
The inorganic phosphate and phos-phocreatine in guinea pig brain were determined after Ca(OH)2-ethanol separation. Both phosphates and creatine were estimated. Inorganic phosphate and phosphates with the lability of phospho-creatine were determined without chemical separation. After death, a very rapid fall in phosphocreatine and rise in inorganic phosphate takes place in the brain of guinea pig as of other species. On slicing brain cortex and allowing the slices to respire with glucose under normal metabolic conditions, the inorganic phosphate falls and the phosphocreatine rises until the concns. approach those normal to the tissue in vivo. The concns. reached vary with the metabolic mixture.