Cinnamic acid derivatives as matrices for ultraviolet laser desorption mass spectrometry of proteins.
Cinnamic acid derivatives as matrices for ultraviolet laser desorption mass spectrometry of proteins.
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DOI：
10.1002/rcm.1290031207
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发表时间：
1989-12-01
期刊：
影响因子：
--
通讯作者：
Chait, B T
中科院分区：
文献类型：
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作者：
Beavis, R C;Chait, B T
本文报道了三种用于基质辅助激光解吸蛋白质的新基质的发现。这些新的基质（辛子酸、阿魏酸和咖啡酸）是肉桂酸衍生物，与以前使用的烟酸基质相比，具有几个实际优势。这些材料在蛋白质准分子离子信号中形成的光化学生成的加合物峰强度要小得多，并且存在的加合物峰更容易分辨。这些基质从目前所检测的所有蛋白质（超过50种）中产生强烈的质子化分子离子。这些新矩阵在真空中也非常稳定，允许它们在非常高真空的应用中方便地使用（例如，傅里叶变换离子回旋共振质谱）。
The paper reports the discovery of three new matrices for the matrix-assisted laser desorption of proteins. These new matrices (sinapinic, ferulic and caffeic acids) are cinnamic acid derivatives that have several practical advantages over the nicotinic acid matrices previously used. These materials form much less intense photochemically generated adduct peaks in the protein quasimolecular ion signal and the adduct peaks that are present are easier to resolve. These matrices produce intense protonated-molecule ions from all of the proteins (over 50) so far examined. These new matrices are also very stable in a vacuum, allowing for their convenient use in very high vacuum applications (e.g., Fourier transform ion cyclotron resonance mass spectrometry).