Comparative proteomic analysis in children with idiopathic short stature (ISS) before and after short-term recombinant human growth hormone (rhGH) therapy
Comparative proteomic analysis in children with idiopathic short stature (ISS) before and after short-term recombinant human growth hormone (rhGH) therapy
复制标题
DOI：
10.1002/pmic.201200131
复制
发表时间：
2013-04-01
期刊：
影响因子：
3.4
通讯作者：
Yoo, Han-Wook
中科院分区：
文献类型：
--
作者：
Heo, Sun Hee;Choi, Jin-Ho;Yoo, Han-Wook
本研究采用蛋白质组学分析方法，通过短期重组人生长激素（rhGH）治疗特发性身材矮小（ISS）患者，确定生长激素（GH）应答蛋白和蛋白表达模式。17名患有ISS的儿童（14男3女）被纳入研究。用剂量为0.31 +/- 0.078 mg/kg/周的rhGH治疗3个月。采用免疫去除6种高富集的血清蛋白，然后进行2D DIGE分析，随后使用MALDI TOF MS，生成短期rhGH治疗后差异表达的蛋白质面板，并验证rhGH治疗后特异性蛋白质的血清水平差异。经rhGH处理后，有14个斑点出现差异表达。其中，apo E和apo L-1表达持续增强，而血清淀粉样蛋白A在rhGH治疗后降低。随后使用rhGH治疗前后的血清进行Western blot分析，验证这些蛋白的差异表达。本研究提示，rhGH治疗可影响ISS患者的脂蛋白代谢，提高载脂蛋白L-1蛋白的表达。
This study was undertaken to identify growth hormone (GH) responsive proteins and protein expression patterns by short-term recombinant human growth hormone (rhGH) therapy in patients with idiopathic short stature (ISS) using proteomic analysis. Seventeen children (14 males and three females) with ISS were included. They were treated with rhGH at a dose of 0.31 +/- 0.078 mg/kg/week for 3 months. Immunodepletion of six highly-abundant serum proteins followed by 2D DIGE analysis, and subsequent MALDI TOF MS, were employed to generate a panel of proteins differentially expressed after short-term rhGH therapy and verify the differences in serum levels of specific proteins by rhGH therapy. Fourteen spots were differentially expressed after rhGH treatment. Among them, apo E and apo L-1 expression were consistently enhanced, whereas serum amyloid A was reduced after rhGH therapy. The differential expressions of these proteins were subsequently verified by Western blot analysis using sera of the before and after rhGH treatment. This study suggests that rhGH therapy influences lipoprotein metabolism and enhances apo L-1 protein expression in ISS patients.