The divalent strontium salt S12911 enhances bone cell replication and bone formation in vitro
The divalent strontium salt S12911 enhances bone cell replication and bone formation in vitro
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DOI：
10.1016/8756-3282(96)00080-4
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发表时间：
1996-06-01
期刊：
影响因子：
4.1
通讯作者：
Marie, PJ
中科院分区：
文献类型：
--
作者：
Canalis, E;Hott, M;Marie, PJ
在这项研究中，我们已经确定了二价锶盐S12911对骨细胞复制和骨形成在两个培养系统的影响。在第一系列实验中，新生大鼠的半个颅骨用S12911培养24 - 96 h，并用H-3-胸苷标记培养的最后6 h，或用S12911处理24 h，并用H-3-脯氨酸标记24 h，然后在去除试剂后24-48 h处理颅骨进行组织形态计量学处理，10（-3）M S12911处理24 h后使前成骨细胞的复制增加30-50%，处理96 h后增加60%。这种效应是特异性的，因为标记的成骨细胞和骨膜细胞的数量没有改变。用10（-3）M的S12911短暂处理24小时，在去除试剂后24和48小时增加骨形成，H-3-脯氨酸标记的表面和骨形成率增加20%-35%。在第二系列实验中，使用连续胶原酶消化法从22天胎鼠颅骨分离富含成纤维细胞或成骨细胞（Ob）的细胞群。在富含成纤维细胞和前成骨细胞的细胞群中，用10（-3）M的S12911处理24 h可使DNA合成增加3 - 4倍。在Ob细胞中的作用不太明显，也不一致，10（-3）M的S12911作用24小时也使Ob细胞中胶原和非胶原蛋白的合成增加35%。这些数据表明，二价锶盐S12911在体外增强骨细胞复制和骨形成，这一作用可能有助于先前报道的S12911在体内对小梁骨量的作用。
In this study, we have determined the effect of the divalent strontium salt S12911 on bone cell replication and bone formation in two culture systems. In the first series of experiments, half-calvariae of newborn rats were cultured with S12911 from 24 to 96 h and labeled with H-3-thymidine for the last 6 h of culture or treated with S12911 for 24 h and labeled for 24 h with H-3-proline 24-48 h after the removal of the agent, Calvariae were then processed for histomorphometry, S12911 at 10(-3) M increased the replication of preosteoblastic cells by 30-50% after 24 h and by 60% after 96 h of treatment. This effect was specific, since the number of labeled osteoblasts and of periosteal cells was not changed, A transient 24 h treatment with S12911 at 10(-3) M increased bone formation 24 and 48 h after the removal of the agent, H-3-proline labeled surfaces and bone formation rates were increased by 20%-35%. In the second series of experiments, sequential collagenase digestions were used to isolate cell populations enriched in fibroblasts or osteoblasts (Ob) from 22 day fetal rat calvariae. Treatment with S12911 at 10(-3) M for 24 h enhanced DNA synthesis by three- to fourfold in cell populations enriched in fibroblasts and preosteoblastic cells. The effect was less pronounced and inconsistent in Ob cells, S12911 at 10(-3) M for 24 h also increased collagen and noncollagen protein synthesis by 35% in Ob cells. These data indicate that the divalent strontium salt S12911 enhances bone cell replication and bone formation in vitro, an effect that may contribute to the previously reported effects of S12911 on trabecular bone mass in vivo.