Elevated level of circulating CD4+Helios+FoxP3+ cells in primary Sjogren's syndrome patients
Elevated level of circulating CD4+Helios+FoxP3+ cells in primary Sjogren's syndrome patients
复制标题
原发性干燥综合征患者循环CD4 Helios FoxP3细胞水平升高
DOI：
10.1080/14397595.2016.1226470
复制
发表时间：
2017-01-01
影响因子：
2.2
通讯作者：
Wang, Hui
中科院分区：
文献类型：
--
作者：
Liu, Chen;Guan, Zhao;Wang, Hui
目的：为探讨调节性T细胞在原发性干燥综合征（pSS）中的表达及CD 4（+）Helios（+）FoxP 3（+）细胞在pSS中的临床意义，采用流式细胞术检测39例pSS患者和30例健康对照者外周血CD 4（+）FoxP 3（+）T细胞，同时检测CD 25和Helios的表达。体外检测CD 4（+）CD 25（hi）细胞的抑制能力。回顾性收集pSS患者的临床资料，分析其与循环Treg细胞的相关性。结果：pSS患者外周血中FoxP 3（+）和Helios（+）FoxP 3（+）细胞水平均高于对照组; CD 4（+）CD 25（hi）细胞的抑制功能在两组之间没有差异。Helios（+）FoxP 3（+）百分率与ESR、IgG、IgM、ESSDAI呈负相关。抗SSB-患者比抗SSB（+）患者具有更高水平的Helios（+）FoxP 3（+）细胞。结论：pSS患者外周血Helios（+）FoxP 3（+）细胞水平升高，可能参与了pSS患者自身免疫功能的抑制。
Objective: This study was designed to investigate the expression of regulatory T cells in primary Sjogren's Syndrome (pSS) and to evaluate the clinical role of CD4(+)Helios(+)FoxP3(+) cells in pSS patients.Methods: CD4(+)FoxP3(+)T cells in the peripheral blood of 39 pSS patients and 30 healthy controls were measured by flow cytometry and CD25 and Helios expression were also analyzed. The repression ability of CD4(+)CD25(hi) cells was tested in vitro. Clinical information of pSS patients was retrospectively collected and their correlations with circulating Treg cells were analyzed. Cytokine levels in plasma were measured by ELISA and correlations with Helios(+)FoxP3(+)cells were also detected.Results: Circulating FoxP3(+)and Helios(+)FoxP3(+)cells were elevated in pSS patients compared with controls. The suppression function of CD4(+)CD25(hi) cells is not different between two groups. There are inverse correlations between Helios(+)FoxP3(+)percentage and ESR, IgG, IgM and ESSDAI. Anti-SSB- patients possess higher level of Helios(+)FoxP3(+)cells than anti-SSB(+)patients. IL-6, IFN and IFN levels were increased in pSS plasma and there were positive correlations between the levels of IFN/IFN and percentage of Helios(+)FoxP3(+)cells.Conclusion: Circulating Helios (+)FoxP3(+) cells were elevated in pSS patients and may contribute to suppressing autoimmunity in pSS patients.