Expression of myelin-associated glycoprotein isoforms after sciatic nerve crush injury in mice.
Expression of myelin-associated glycoprotein isoforms after sciatic nerve crush injury in mice.
复制标题
DOI：
10.1007/bf03160072
复制
发表时间：
1993-12
期刊：
影响因子：
--
通讯作者：
T. Inuzuka;S. Sato;N. Fujita;H. Baba;R. Nakano;H. Ishiguro;T. Miyatake
中科院分区：
文献类型：
--
作者：
T. Inuzuka;S. Sato;N. Fujita;H. Baba;R. Nakano;H. Ishiguro;T. Miyatake
在小鼠坐骨神经挤压损伤后，通过分别使用针对 L-MAG 蛋白和 S-MAG 蛋白的特异性抗体的免疫印迹技术，证实了大髓磷脂相关糖蛋白同种型 (L-MAG) 蛋白和小髓磷脂相关糖蛋白同种型 (S-MAG) 蛋白。免疫印迹表明，压碎的坐骨神经中两种异构体蛋白的表达迅速下降至对照侧的 <10%。损伤后13天，L-MAG蛋白表达迅速恢复至对照水平的100%。随着L-MAG蛋白表达的增加，S-MAG蛋白表达在损伤后20天恢复到100%。据报道，在中枢和周围神经系统（CNS和PNS）中，L-MAG蛋白的发育最大表达先于S-MAG蛋白。我们之前的工作表明，L-MAG mRNA 在最活跃的髓鞘形成（包括中枢神经系统中的髓鞘再生）时被特征性地诱导。我们在此表明，在三七总皂苷活跃的髓鞘再生过程中，L-MAG 蛋白的表达先于 S-MAG 蛋白的表达。这表明它在髓磷脂形成的早期阶段发挥着重要作用。
The large myelin-associated glycoprotein isoform (L-MAG) protein and small myelin-associated glycoprotein isoform (S-MAG) protein were demonstrated after sciatic nerve crush injury in mice by an immunoblotting technique using specific antibodies to the L-MAG protein and the S-MAG protein, respectively. Immunoblots indicated a rapid decrease in expression of both isoform proteins in the crushed sciatic nerves to <10% of the control side. By 13 d after injury, L-MAG protein expression had quickly recovered to 100% of the control level. Following the increase in L-MAG protein expression, S-MAG protein expression recovered to 100% by 20 d after injury. It has been reported that the developmental maximum expression of L-MAG protein precedes that of S-MAG protein in both central and peripheral nervous system (CNS and PNS). Our previous work demonstrated that L-MAG mRNA was characteristically induced at the time of most active myelination, including remyelination in the CNS. We here have shown the expression of L-MAG protein precedes that of S-MAG protein during active remyelination in the PNS. This suggests that it plays an important role in the early stage of myelin formation.