A MODEL-FREE APPROACH TO ESTIMATION OF RELATIVE POTENCY IN DOSE-RESPONSE CURVE ANALYSIS
A MODEL-FREE APPROACH TO ESTIMATION OF RELATIVE POTENCY IN DOSE-RESPONSE CURVE ANALYSIS
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DOI：
10.1152/ajpendo.1987.252.3.e357
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发表时间：
1987-03-01
影响因子：
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通讯作者：
MUNSON, PJ
中科院分区：
文献类型：
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作者：
GUARDABASSO, V;RODBARD, D;MUNSON, PJ
我们已经发展了一种新的，通用的方法来分析剂量-反应曲线的生物测定，免疫测定（包括放射免疫测定，免疫放射分析，酶联免疫吸附测定），和其他实验程序。它提供了一个测试的平行性，形状的相似性，并衡量相对效力的任何一组两个或多个曲线。该方法使用约束平滑样条函数估计曲线形状，并使用非线性最小二乘拟合技术估计相对效价和斜率的参数。使用“约束样条”允许分析不能用简单模型或方程（如对称四参数逻辑）描述的非线性剂量-反应曲线。使用微型计算机程序进行分析，提供相对效价及其SE和拟合优度评价。
We have developed a new, general approach to analysis of dose-response curves from bioassay, immunoassay (including radioimmunoassay, immunoradiouretic assay, enzyme-linked immunosorbent assay), and other experimental procedures. It provides a test for parallelism, similarity of shape, and a measure of relative potency for any set of two or more curves. The method uses a constrained smoothing spline function to estimate the curve shape, together with a nonlinear least-squares fitting technique to estimate parameters for relative potency and slope. The use of "constrained splines" permits that analysis of nonlinear dose-response curves that cannot be described by a simple model or equation such as the symmetric four-parameter logistic. A microcomputer program is used for the analysis, providing relative potencies and their SE and evaluation of goodness of fit.