Molecular characterization of Prototheca strains isolated from Italian dairy herds
Molecular characterization of Prototheca strains isolated from Italian dairy herds
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DOI：
10.3168/jds.2010-3178
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发表时间：
2010-10-01
影响因子：
3.5
通讯作者：
Buzzini, P.
中科院分区：
文献类型：
--
作者：
Ricchi, M.;Goretti, M.;Buzzini, P.
采用基因型特异性PCR方法，对意大利24头奶牛的复合奶和棚周环境样品（床上用品、粪便、饮水或洗涤水、表面拭子）中分离到的161株原鞘菌进行了鉴定。总体而言，97.2%的复合乳样品分离到的菌株被鉴定为zopfii基因2型，证实其是主要的乳腺炎病原体，而blaschkeae仅零星分离到（2.8%）。在环境采样中，84.9%的分离株为zopfip基因2型，13.2%为blaschkeae， 1.9%为zopfip基因1型。本研究的数据与之前的假设相矛盾，之前的假设认为2型zopfii基因型是原鞘性乳腺炎的唯一病原体，相反；在少数情况下，证实了这种病理可能是由blaschkeae引起的假设。
One hundred sixty-one Prototheca spp. strains isolated from composite milk and barn-surrounding environmental samples (bedding, feces, drinking, or washing water, surface swabs) of 24 Italian dairy herds were characterized by genotype-specific PCR analysis. Overall, 97.2% of strains isolated from composite milk samples were characterized as Prototheca zopfii genotype 2, confirming its role as the main mastitis pathogen, whereas Prototheca blaschkeae was only sporadically isolated (2.8%). Regarding environmental sampling, 84.9% of isolates belonged to P. zopfii genotype 2, 13.2% to P. blaschkeae, and 1.9% to P. zopfii genotype 1. The data herein contradict previous hypotheses about the supposed exclusive role of P. zopfii, genotype 2 as the causative agent of protothecal mastitis and, on the contrary; confirm the hypothesis that such pathology could be caused by P. blaschkeae in a few instances.