COMPARISON OF HENSEN NODE AND RETINOIC ACID IN SECONDARY AXIS INDUCTION IN THE EARLY CHICK-EMBRYO
COMPARISON OF HENSEN NODE AND RETINOIC ACID IN SECONDARY AXIS INDUCTION IN THE EARLY CHICK-EMBRYO
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DOI：
10.1002/aja.1001950209
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发表时间：
1992-10-01
影响因子：
2.5
通讯作者：
SOLURSH, M
中科院分区：
文献类型：
--
作者：
CHEN, YP;SOLURSH, M
维甲酸(RA)和汉森结节是鸡胚胎中的组织者中心，已被证明在应用或嫁接到鸡肢芽中时具有极化活性。在这里，我们调查和比较了RA和移植的Hensen‘s结节对早期鸡胚的影响。用5~100 ng/mlRA浸泡的阴离子交换小球植入4期鸡卵胚胎宿主前后轴的前侧或左侧均能诱导次级轴的形成，而相同浓度的RA浸泡的微球植入宿主轴的右侧或后侧则不能诱导次级轴的形成。所有的诱导轴都含有树干-尾巴结构。将来自鹌鹑胚胎的Hensen‘s结节植入早期鸡胚层，除了移植物自身分化为第二轴外，还可能导致第二轴的形成。RA和移植的Hensen‘s结节均导致前脑发育抑制，后脑发育增加，宿主心脏向异常方向循环。这些结果支持Hensen‘s结节是RA的来源的假说，RA与早期胚胎发生有关。另外，RA可能刺激邻近组织形成Hensen‘s结节特性。
Retinoic acid (RA) and Hensen's node, the organizer center in the chick embryo, have been shown to have polarizing activity when applied or grafted into the chick limb bud. Here we investigate and compare the effects of RA and grafted Hensen's node on the early chick embryo. Anion exchange beads soaked with RA at concentrations ranging from 5 to 100 ng/ml and implanted on the anterior side or on the left side of the host anteroposterior axis of a stage 4 chick embryo in ovo have the ability to induce secondary axis formation, while beads soaked with RA of the same concentration and implanted on the right side or on the posterior side of the host axis are unable to induce the secondary axis. All of the induced axes contain trunk-tail structures. Hensen's node from quail embryos implanted into the early chick blastoderm could also cause the formation of secondary axes in addition to self-differentiation of the graft into a secondary axis. Both RA and grafted Hensen's node caused the inhibition of forebrain development with an increase in hindbrain development and the host heart to loop in an abnormal direction. The results support the hypothesis that Hensen's node is a source of RA which is involved in early embryogenesis. Alternatively, RA might stimulate the formation of Hensen's nodal properties in adjacent tissue.