Immunohistochemical Localization of Six Galectin Subtypes in the Mouse Digestive Tract
Immunohistochemical Localization of Six Galectin Subtypes in the Mouse Digestive Tract
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DOI：
10.1369/jhc.2008.952317
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发表时间：
2009-01-01
影响因子：
3.2
通讯作者：
Iwanaga, Toshihiko
中科院分区：
文献类型：
--
作者：
Nio-Kobayashi, Junko;Takahashi-Iwanaga, Hiromi;Iwanaga, Toshihiko
半乳糖凝集素是一种能识别糖缀合物中β-半乳糖苷的动物凝集素，在肠道中含量丰富。本研究显示了半乳糖凝集素在小鼠消化道中的亚型特异性定位。粘液上皮显示每个半乳糖凝集素亚型的区域/细胞特异性定位。胃粘膜细胞半乳糖凝集素-2和半乳糖凝集素-4/6表现出强烈的免疫反应，半乳糖凝集素-3在胃粘膜表面的有限定位。电镜下，胃粘膜细胞上有galectin-3免疫反应阳性的土著细菌。小肠上皮细胞中的半乳糖凝集素-2和galectin-4/6的特征定位在杯状细胞的细胞质和肠上皮细胞的基底外侧膜与成熟，分别。半乳糖凝集素-3只在绒毛尖端表达，集中在肌球蛋白丰富的完全成熟的肠上皮细胞的终端网络。大肠上皮细胞含有强烈的免疫反应半乳糖凝集素-3和半乳糖凝集素-4/6，但不半乳糖凝集素-2。前胃复层鳞状上皮半乳糖凝集素-3和半乳糖凝集素-7免疫反应，但基底层缺乏半乳糖凝集素-3免疫反应。在上皮外，只有半乳糖凝集素-1定位于结缔组织、平滑肌和神经元细胞体中。半乳糖凝集素的亚型特异性定位表明其在宿主-病原体相互作用和上皮内稳态中的重要作用，如膜极化和在肠道中的运输。（组织化学细胞化学杂志57：41-50，2009）
Galectin, an animal lectin that recognizes beta-galactoside of glycoconjugates, is abundant in the gut, This IHC study showed the subtype-specific localization of galectin in the mouse digestive tract. Mucosal epithelium showed region/cell-specific localization of each galectin subtype. Gastric mucous cells exhibited intense immunoreactions for galectin-2 and galectin-4/6 with a limited localization of galectin-3 at the surface of the gastric mucosa. Electron microscopically, galectin-3 immunoreactivity coated indigenous bacteria on the gastric surface mucous cells. Epithelial cells in the small intestine showed characteristic localizations of galectin-2 and galectin-4/6 in the cytoplasm of goblet cells and the baso-lateral membrane of enterocytes in association with maturation, respectively. Galectin-3 expressed only at the villus tips was concentrated at the myosin-rich terminal web of fully matured enterocytes. Epithelial cells of the large intestine contained intense immunoreactions for galectin-3 and galectin-4/6 but not for galectin-2. The stratified squamous epithelium of the forestomach was immunoreactive for galectin-3 and galectin-7, but the basal layer lacked galectin-3 immunoreactivity. Outside the epithelium, only galectin-1 was localized in the connective tissue, smooth muscles, and neuronal cell bodies. The subtype-specific localization of galectin suggests its important roles in host-pathogen interaction and epithelial homeostasis such as membrane polarization and trafficking in the gut. (J Histochem Cytochem 57:41-50, 2009)